摘要
目的:观察保存温度及离心管的材质在制备富血小板纤维体外释放生长因子中发挥的作用,优化富血小板纤维蛋白的提取方法。方法:采集志愿者的静脉血,分别使用玻璃材质的离心管和塑料材质的离心管进行离心,制备完成后将收集到的PRF分别置于4℃和37℃的环境中进行保存,收集PRF所释放的生长因子,比较不同方法得到的富血小板纤维蛋白释放的血小板衍生生长因子-AB(platelet-derived growth factor,PDGF-AB)、转化生长因子-β(transforming growth factor-β,TGF-β)的量的差异。结果:使用玻璃试管组的PRF释放的PDGF-AB和TGF-β的量高于对照组,存放于4℃条件的PRF释放的生长因子高于对照组。结论:在制备PRF时,建议使用玻璃材质的离心管储存于4℃左右的环境中,以便PRF发挥更好地促进组织生长于修复的作用。
Objective： Observation of preservation temperature and centrifugal pipe material in the prepara-tion of platelet rich fiber in vitro release of growth factors play a role ,to optimize the extraction methods of rich platelet fibrin. Method： Venous blood collection and volunteers, using glass centrifugal cen-trifuge tube and plastic centrifuge tube, after the completion of the preparation of the collected PRF re-spectively at 4 ℃ and 37 ℃ to save, collect PRF release of growth factors, to compare different ways to get rich platelet fibrin release of platelet-derived growth factor-AB （platelet-derived growth factor, PDGF-AB）, transforming growth factor beta （transforming growth factor beta, TGF-beta） the amount of difference. Result：Use glass tube group PRF release PDGF-AB and TGF-the amount of beta is higher than the control group, stored at 4℃condition of PRF release of growth factor is higher than the control group. Conclusion： In the preparation of PRF, it is recommended to use glass centrifugal pipe stored at 4℃about environment, in order to better promote tissue growth PRF play to the role of the left.
出处
《中国口腔种植学杂志》
2016年第1期6-8,共3页
Chinese Journal of Oral Implantology
基金
吉林省科技厅科研课题(2012-5053)
关键词
温度
PRF
生长因子
玻璃离心管
塑料离心管
temperature
PRF
growth factor
glass centrifugal tube
plastic centrifuge tube
