摘要
目的观察当归红芪超滤膜提取物对过氧化氢(H2O2)致PC12细胞凋亡的影响,探讨其作用机制。方法 H2O2诱导PC12细胞凋亡,构建氧化损伤神经细胞凋亡模型。实验分为正常对照组、模型组及中药低、中、高剂量组,中药各组给予不同剂量当归红芪超滤膜提取物。流式细胞术检测细胞凋亡率,激光共聚焦显微镜检测细胞线粒体膜电位变化,Western blot检测Bax、Bcl-2和cleaved Caspase-3的蛋白表达。结果与模型组比较,中药各剂量组细胞凋亡率明显降低,线粒体膜电位升高,Bax、cleaved Caspase-3蛋白表达均减弱,Bcl-2表达增强,Bcl-2/Bax比值升高(P<0.05)。结论当归红芪超滤膜提取物具有抗H2O2诱导的PC12细胞凋亡的作用。
Objective To observe the effects of ultrafiltration extract from Angelica Sinensis Radix and HedysariRadix on PC12 cell apoptosis induced by H2O2; To discuss its mechanism of action. Methods H2O2 was used in theincubation of PC12 cells to establish the oxidative damage nerve cell apoptosis model. The experiment was dividedinto normal control group, model group, and three different dosages （0.375, 0.75, 1.5 g/L） of ultra-filtration extractedfrom Angelica Sinensis Radix and Hedysari Radix after modeling for interference. Rate of cell apoptosis was detectedby flow cytometry; mitochondrial membrane potential was measured by laser scanning confocal microscopy; theprotein expressions of cleaved Caspase-3, Bax and Bcl-2 were detected by Western blot. Results Compared with themodel group, rates of cell apoptosis in the different dosage groups decreased significantly; membrane potential ofmitochondria increased; the protein expressions of cleaved Caspase-3 and Bax decreased; the expression of Bcl-2increased; the ratio of Bcl-2 and Bax increased （P〈0.05）. Conclusion The ultra-filtration extracted from AngelicaSinensis Radix and Hedysari Radix can inhibit PC12 cell apoptosis induced by H2O2.
出处
《中国中医药信息杂志》
CAS
CSCD
2016年第6期68-72,共5页
Chinese Journal of Information on Traditional Chinese Medicine
基金
国家自然科学基金(81160478)
甘肃省高等院校研究生导师科研项目(BH2011-043)
关键词
当归红芪超滤膜提取物
过氧化氢
PC12细胞
凋亡
ultra-filtration extract from Angelica Sinensis Radix and Hedysari Radix
H2O2
PC12 cells
apoptosis
