摘要
目的探索大蒜素对小鼠胸腺淋巴细胞亚群分化的影响。方法将80只清洁级昆明小鼠随机分为空白对照组和大蒜素低剂量组、中剂量组、高剂量组,每组20只,每天灌胃1次,灌胃容量为0.1ml/10g,连续灌胃14d。1周后将小鼠处死,分离胸腺计算脏器指数并制取单细胞悬液,用流式细胞仪检测分析大蒜素对各组小鼠胸腺淋巴细胞CD4^+T细胞、CD8^+T细胞及CD4^+/CD8^+值的影响。结果空白对照组和各剂量组小鼠的胸腺指数、胸腺淋巴细胞CD4^+T细胞和CD8^+T细胞值有差异(F=6.82,F_(CD4^+)=14.12,F_(CD8^+)=8.27,P<0.05),经两两比较空白组、低剂量组、中剂量组与高剂量组4组之间小鼠的胸腺指数、胸腺淋巴细胞CD4^+T细胞和CD8^+细胞值均有较显著差异(P<0.05),各剂量组之间的CD4^+/CD8^+值无差异(F=2.44,P>0.05)。结论大蒜素使小鼠的胸腺指数、胸腺淋巴细胞CD4^+T细胞和CD8^+T细胞比例均升高,且存在剂量依赖性,对小鼠免疫力具有正向调节作用。
Objective To explore the effects of allicin on T lymphocyte subsets of thymus in mice. Methods Eighty healthy adult Kunming mice were randomly divided into 4 groups including control group and allicin Low-dose group,Mid-dose group,High-dose group. Each group of 20 mice were treated with allicin by gavage,0. 1ml /（ 10 g﹒bw） once every day for 14 days which were killed after a week. The thymus was collected to content the organ index then making single cell suspensions. FCM was used to study the effects of allicin on the percentage of CD4^＋,CD8^＋,CD4^＋/ CD8^＋lymphocyte of thymus. Results The thymus index,thymus lymphocytes CD4^＋T cells and CD8^＋T cells of all dose groups and the control group mice were all significant differences（ F = 6. 82,F（CD4 ^＋）= 14. 12,F（CD8 ^＋）= 8. 27,P〈0. 05）.And they had significant difference among the three dose and control groups by pairwise comparisons（ P〈0. 05）. It had no difference effects on the percentage of CD4^＋/ CD8^＋lymphocyte of thymus（ F = 2. 44,P〉0. 05）. Conclusion The thymus index and thymus lymphocytes CD4^＋T cells CD8^＋T cells of all-dose group mice were increased in a dose-dependent effect in mice. Allicin has a positive regulation on the immunity of mice.
出处
《济宁医学院学报》
2016年第2期87-90,共4页
Journal of Jining Medical University
基金
济宁市科技发展计划项目(2013JNWK74)
