摘要
目的研究水提乌药与醇提乌药对急性酒精性肝损伤模型大鼠的保护作用。方法将健康雄性SD大鼠按照体重随机分为4组:正常组、模型组、水提乌药组(WYSTW)、醇提乌药组(WYCTW),每组10只。正常组,灌胃等体积蒸馏水),其余各组,均梯度灌胃给予不同浓度的白酒,每日2次,灌胃容积10m L·kg^(-1),连续10 d造模结束。自造模第l天起,给药组(均1 g·mL^(-1))分别灌胃给予相应的药物,每日1次,灌胃容积为10 m L·kg^(-1)体重,连续10 d。用全自动生化仪测定血清总胆固醇(TC)、三酰甘油(TG)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、血清超氧化物歧化酶(SOD)、丙二醛(MD)水平;观察大鼠肝病理学改变,免疫组化法观察肝组织中肿瘤坏死因子-α(TNF-α)、核转录因子-κB(NF-κB)、白细胞介素-1β(IL-1β)的表达。结果与模型组比较,水提乌药组、醇提乌药组均能有效降低模型大鼠血清ALT、AST、MDA、TG含量(P<0.05);升高模型大鼠血清及肝组织SOD活性(P<0.05);能不同程度地改善模型大鼠肝组织病理学改变,抑制肝细胞中IL-1β、NF-κB、TNF-α的表达。结论水提乌药和醇提乌药组有不同程度的抗炎、抗氧化及保护肝细胞的作用。
Objective To observe the protective effect of the water extract and alcohol extract of Radix Linderae on acute alcoholic liver injury in rats. Methods Forty healthy male SD rats were randomly divided into four groups： normal group（n = 10）,model group（n = 10）,the water extract of Radix Linderae group（WYSTW,n = 10）,and the alcohol extract of Radix Linderae group（WYCTW,n = 10）. The normal group was given equal volume of water,while the other groups were given different concentrations of alcohol,twice a day,and company with the WYSTW and WYCTW（both 1 g · mL^-1） for 10 days. Serum total cholesterol（TC）,triglyceride（TG）,alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,serum superoxide dismutase（SOD）,malondialdehyde（MDA） were measured. The expression of interleukin-1 beta（IL-1β）,nuclear transfer factor kappa B（NF-κB） and tumor necrosis factor-α（TNF-α） in liver tissue was observed by immuno histochemical method. Results Compared with model group,Radix Linderae extracts can effectively reduce the serum ALT,AST,MDA,TG contents（P〈0. 05）,and effectively increase the SOD activity in serum and livertissue（P〈0. 05）. WYSTW and WYCTW can improve liver tissue pathological change and control expression of IL-1 β,NF-κB,TNF-α in liver cells. Conclusion WYSTW and WYCTW have the protective effect on acute alcoholic liver injury in rats and have the effect of anti-inflammatory and anti-oxidation.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2016年第8期703-706,共4页
The Chinese Journal of Clinical Pharmacology
基金
浙江省中医药局基金资助项目(2008CA117)
浙江省科技厅公益性技术应用研究计划基金资助项目(2013C33222)
浙江省医药卫生科技计划基金资助项目(2015DTA021)
关键词
乌药
醇提取物
水提取物
肝损伤
核转录因子-ΚB
Radix Linderae
alcohol extract
water extract
liver injury
nuclear factor kappa B
