摘要
为获取高生物活性绿原酸,利用金属-酶共催化处理金银花叶,采用响应曲面法对酶投加量、酶解温度、酶解时间和pH值等影响绿原酸溶浸的因素进行优化,并分析因素间交互作用。结果表明,各因素对绿原酸溶浸的影响顺序为pH值>时间>温度>酶量;绿原酸提取最佳工艺条件为:复合酶(锰锌铁氧体∶纤维素酶∶漆酶∶木聚糖酶=21∶43∶35∶1)0.79g,pH值3.98,水浴温度41.2℃,共催化时间5.3h,该条件下,2.0g金银花叶溶浸绿原酸质量浓度为4.02mg/g,得率为(4.16±0.14)mg/g,为提高高附加值绿原酸的工业生产提供技术支撑。
To obtain high biological activity of chlorogenic acid,the extraction of chlorogenic acid from lonicera japonica leaf by metal-enzyme catalytic hydrolysis was optimized by response surface methodology,the effects of enzyme dosage,hydrolysis temperature,extraction time and p H on the extraction yield of chlorogenic acid and interactions between these factors were investigated. The results showed that four parameters of their effect on the extraction yield of chlorogenic acid were ranked as follows： p H extraction time hydrolysis temperature enzyme dosage; the optimal condition of chlorogenic acid were found to be0. 79 g compound enzymes,p H 3. 98,41. 2 ℃ and 5. 3 h. The mass concentration of chlorogenic acid under the optimized conditions was 4. 02 mg / g,the yield was（ 4. 16 ± 0. 14） mg / g.
出处
《应用化工》
CAS
CSCD
北大核心
2016年第4期707-710,共4页
Applied Chemical Industry
基金
重庆市应用开发重点项目资助(cstc2013yykf B20001)
关键词
绿原酸
金属-酶共催化
响应曲面分析
工艺
chlorogenic acid
metal-enzyme catalysis
response surface methodology
process
