摘要
目的研究从怀枝果皮中提取原花青素的最佳条件。方法以怀枝果皮作为提取原花青素的原料进行提取条件的单因素试验,研究的因素包括超声功率、提取时间、乙醇比例、提取温度、料液比例、p H值,然后以此为依据进行L27(35)正交试验得出最佳提取条件。结果研究表明从怀枝果皮提取原花青素的最佳条件是以p H值6.0的70%乙醇溶液为提取溶剂,温度为50℃、料液比例为1∶15,超声功率为400 W提取40 min,并重复提取2次。各因素对原花青素提取效果的影响大小依次为:超声功率>提取时间>提取温度>乙醇比例>料液比例。结论优化后的提取条件能有效提高从怀枝果皮中提取原花青素的效率。
OBJECTIVE To study the most effective condition of extracting procyanidins from Litchi pericarp （Huaizhi）. METHODS Single factor experiments of extracting procyanidins from Litchi pericarp （Huaizhi） was carried out including ultrasonic power, extracting period, ethanol percentage, solvent pH, solid/liquid ratio and temperature. Orthogonal array design L27 （35） was carried out to find the most effective condition of extracting procyanidins. RESULTS The results showed that the most optimal extraction were found to be applying pH 6.0 70% ethanol to extract twice in 400 W ultrasonic power at 50 ℃ for 40 min with a solid/ liquid ratio of 1 ： 15. The effect of 5 important variables on the extracting rate of proanthocyanidins followed decreasing order： uhrasonic power 〉 extracting period 〉 temperature 〉 ethanol percentage 〉 solid/liquid ratio. CONCLUSION Proanthocyanidins could be extracted more effectively from Litchi pericarp via the optimized extraction.
出处
《今日药学》
CAS
2016年第3期165-168,共4页
Pharmacy Today
关键词
怀枝
果皮
原花青素
正交试验
Huaizhi
pericarp
procyanidins
orthogonal test
