摘要
[目的]研究肝苏片槲皮素的提取工艺,优选最佳提取条件。[方法]在确定槲皮素对照品线性范围的条件下,以槲皮素的含量为指标,考察一定范围的水浴温度以及提取溶剂的酸度对肝苏片槲皮素收率的影响,优选出最佳提取工艺条件。再对优选后的条件进行精密度试验、稳定性试验、回收率试验以及多批样品检测试验等方法学验证,最终确定该条件的适用性和正确性。[结果]最佳槲皮素提取工艺是在1.5%的盐酸甲醇作为溶剂、60℃作为水浴回流的温度条件下,槲皮素含量为1.05 mg/片。[结论]该方法准确可靠、含量高、波动范围小,可作为肝苏片的含量测定标准参考。
[Objective]The research aimed to study the extraction technology of quercetin from gansu pill,and to optimize the extraction conditions.[Method]Under the condition of determining the linear range of quercetin reference substance, the content of quercetin is used as an indicator, the effects of water bath temperature and acidity of extraction solvent on the yield of quercetin in Gansu tablets were investigated and the optimum extraction conditions were selected. Then, under the above optimum conditions, the precision test, stability test, recovery test and multi-batch sample test were carried out to verify these results. Finally, we get the applicability and correctness of the above conditions.[Result]The optimal quercetin extraction process was quercetin content of 1.05 mg/tablet, when the concentration of methanol was 1.5% and the bath temperature was 60 ℃.[Conclusion]The method is accurate and reliable with high content and small fluctuation range, it can be used as the standard for the determination of gansu pill.
作者
罗倩
孙雁霞
赵彪
邬晓勇
LUO Qian;SUN Yan-xia;ZHAO Biao(College of Pharmaceutical and Biological Engineering,Chengdu University,Chengdu,Sichuan 610106;Sichuan Tianyi University,Deyang,Sichuan 618200;Chengdu Guojia United Pharmaceutical Co. Ltd.,Chengdu,Sichuan 610036)
出处
《安徽农业科学》
CAS
2019年第6期178-180,共3页
Journal of Anhui Agricultural Sciences
关键词
肝苏片
槲皮素
含量测定
高效液相色谱法
Gansu pill
Quercetin
Content determinnation
High performance liquid chromatography
