摘要
目的探讨从石蜡包埋胃镜组织中提取幽门螺杆菌菌体DNA的方法和策略。方法选取2008年5月～2010年5月中国医科大学263例胃癌术后石蜡包埋标本及2011年6月～2014年6月医学院附属中心医院和沈阳医学院附属第二医院206例幽门螺杆菌阳性石蜡包埋标本,利用试剂盒提取菌体DNA、PCR扩增,对扩增的结果进行统计分析。结果 263例胃癌大体标本中,231例检测出随意选取基因片段,阳性率为87.83%;206例胃镜标本中,194例检测出幽门螺杆菌cag A基因,阳性率为89.81%。胃大体标本和胃镜标本DNA提取阳性率之间比较差异无统计学意义（P〉0.05）。结论从石蜡包埋胃镜组织中提取幽门螺杆菌菌体DNA是高效可行的。
Objective To analyze the methods and strategies about helicobacter pylori DNA extraction from Paraffin embedding gastroscope organization. Methods A total of 263 cases of postoperative gastric cancer specimens of paraffin embedding were selected from China Medical University from May 2008 to May 2010. 206 cases of helicobacter pylori positive specimens of paraffin embedding were selected from Shenyang Center Hospital and the Second Hospital affiliated to Shenyang Medical College from June 2011 to June 2014. DNA extraction from helicobacter pylori by FFPE DNA kit,PCR and analyzed the income. Results 263 cases of gastric cancer in specimens, 231 cases detected randomly selected gene amplification, positive rate was 87.83%; Specimens of 206 cases of gastroscope, with 194 cases of helicobacter pylori cag A gene were detected,the positive rate was 89.81%. The differences of the DNA extraction rate between Stomach gross specimens and the gastroscope, without statistically significant difference（P〉0.05）. Conclusion Extracting helicobacter pylori DNA from paraffin embedding gastroscope organization is efficient and feasible.
出处
《中国现代医生》
2016年第8期121-123,F0003,共4页
China Modern Doctor
基金
辽宁省科技计划项目(201202210)
关键词
石蜡包埋组织
幽门螺杆菌
DNA
基因扩增
Paraffin embedding tissue
Helicobacter pylori
DNA
Gene amplification
