摘要
目的探讨辽宁地区胃癌患者幽门螺杆菌(Helicobacter pylori,H.pylori)vacA优势基因型并探讨vacA不同基因亚型与NF-κB表达的关系。方法选取自2010年7月至2014年7月我院手术切除的H.pylori(+)的胃癌组织样本140例。按照试剂盒说明书提取胃癌组织的DNA,采用PCR技术检测H.pylori不同vacA基因型表达情况,以免疫组化方法检测胃癌组织NF-κB蛋白表达。结果在胃癌中vacAs1、vacAm1、vacAs1m1亚型分布高于其他亚型(P <0.05)。其中,vacAm1和vacAs1m1基因型在弥散型胃癌中比例高于肠型胃癌,差异有显著统计学意义(P <0.01);NF-κB表达与性别和淋巴转移情况密切相关,其中NF-κB在男性中表达明显高于女性(P <0.05)。在淋巴结转移(+)的患者中NF-κB的表达明显高于淋巴结转移(-)的患者(P <0.01)。NF-κB表达与H.pylori vacA(s1、m1、s1m1)三种亚型密切相关,并且当此三种基因型为阳性时,NF-κB阳性表达率均明显升高。结论辽宁地区内胃癌发生的优势基因型为vacAm1、vacAs1、vacAs1m1三种亚型,携带vacAm1和vacAs1m1基因亚型的患者可能是弥散型胃癌的高发人群,且其可能通过促进NF-κB的表达进而导致胃癌的发生。
Objective To analyze vacA dominant genotypes of Helicobacter pylori(H.pylori) in gastric cancer patients in Liaoning and its association with NF-κB expression and provide laboratory basis for the mechanism and clinical treatment of gastric cancer.Methods A total of 140 cases of gastric cancer tissue samples with positive H.pylori were determined by surgical resection from 2010 to 2014.DNA was extracted from gastric cancer tissues according to the kit instructions,and the expression of different vacA genotypes of H.pylori was detected by PCR technology.The expression of NF-κB protein in gastric cancer tissues was detected by immunohistochemistryResults The distribution of vacAsl,vacAml,and vacAslml subtypes in gastric cancer was higher than that of other subtypes(P <0.05).Among them,the proportion of vacAml and vacAslml genotypes in diffuse gastric cancer is higher than that of intestinal gastric cancer,and the difference is statistically significant(P <0.01).NF-κB expression is closely related to gender and lymphatic metastasis and the expression of NF-κB in male patients was significantly higher than that in female patients,indicating statistical significance(P <0.05).The expression of NF-κB was significantly higher in patients with lymphatic metastasis than in those,and the difference was statistically significant(P <0.01).NF-κB expression was closely related to H.pylori vacAsl,vacAml,and vacAslml subtypes,and the differences were statistically significant(P <0.05).When these three genotypes were positive,the positive expression rate of NF-κB was significantly increased.Conclusion H.pylori vacAml,vacAsl,and vacAslml are the dominant genotypes of gastric cancer in Liaoning.Patients carrying vacAslml gene subtype may be a high-incidence population of diffuse gastric cancer,and vacAslml gene subtype may promote the expression of NF-κB and cause gastric cancer.
作者
薄威
王旭光
李佳琦
梁伊灵
李磊
勾佳钰
张忠
BO Wei;WANG Xuguang;LI Jiaqi;LIANG Yiling;LI Lei;GOU Jiayu;ZHANG Zhong(Department of Pathology,Shenyang Medical College,Shenyang 110034,China)
出处
《实用医学杂志》
CAS
北大核心
2021年第6期782-786,共5页
The Journal of Practical Medicine
基金
辽宁省科学技术计划项目省自然科学基金指导计划(编号:20170540885)
沈阳医学院青年科学研究基金(编号:20202025)。
关键词
NF-ΚB
幽门螺杆菌
空泡毒素
相关性
胃癌
NF⁃κB
Helicobacter pylori
vacuolar toxin
correlation
gastric cancer
