摘要
目的建立尿液中百草枯的离子色谱检测方法，观察百草枯中毒患者尿液中百草枯浓度的动态变化趋势，为临床救治提供参考依据。方法样品处理：将尿液离心5min（3000r／min），取上清液用微孔滤膜过滤，弃掉初滤液进样。淋洗液10％（体积分数）乙腈与0．05mol／L七氟丁酸的混合液。结果尿液中百草枯线性范围在0．5～8mg／L（r=0．9997），回收率为95．3％-102．1％，RSD为2．6％-3．4％，检出下限为2．3μg/L。结论该方法快速、准确、无干扰，适合于中毒患者尿样中百草枯浓度的监测。
Objective To establish an ion chromatography method for the determination of paraquat in urine and ob- serve the dynamic change of paraquat in the urine of the patients with acute paraquat poisoning. Methods The samples were filtered after centrifugation for five minutes. The eluent was the mixture of 0. 05 mol/L heptafluorobutanoic acid and 10% ACN. Results The linear range between 0. 5 -8 mg/L was good, the recovery was between 95.3% - 102. 1%, and RSD was between 2. 6% - 3.4% , the detection limit was 2. 3μg/L. Conclusion Ion chromatography was rapid and accurate without interference. It is suitable for the analysis of the paraquat in urine.
出处
《现代医院》
2016年第3期381-382,385,共3页
Modern Hospitals
基金
广东省科技计划项目(编号:粤科规划字[2013]137号)
广东省医学科研基金(编号:A2014597)
广州市民生科技重大项目(编号:2012Y2-00029-1)
广东省"十二五"医学重点专科
广州市医学重点学科建设项目
广州市职业环境与健康效应实验室建设项目(编号:2014SY000020)
关键词
离子色谱法
百草枯
尿样
Ion Chromatography
Paraquat
Urine
