摘要
目的研究加味四逆散对抑郁症大鼠海马BDNF、NR1表达的调控及促进海马DG区神经再生的效应及机制。方法建立慢性应激性抑郁症模型。采用荧光标记的免疫组化方法检测海马DG区NeuN、BrdU、脑源性神经营养因子(BDNF)、N-甲基-D-天冬氨酸受体1(NR1)的表达水平。原位杂交技术检测海马DG区BDNF表达水平。结果慢性应激能抑制海马DG前体细胞的增殖(P<0.01);抑郁症大鼠海马DG区BDNF表达明显下降(P<0.01),NR1的表达明显升高(P<0.01)。JWSNS和氟西汀能明显增加抑郁症大鼠海马DG单位面积中神经元数目和新增殖细胞量(P<0.01),增强海马DG区BDNF的表达(P<0.01)和降低NR1的表达(P<0.01)。结论 JWSNS能够促进抑郁症大鼠海马DG区神经细胞的增殖,并可能通过增强BDNF的表达和降低NR1的表达,促进海马DG区的神经再生。
Aim To study the regulatory of Jiaweisinisan on expression of hippocampal BDNF,NR1 and dental gyrus( DG) neurogenesis in rats with chronic stressed-depression and its possible mechamisms.Methods Chronic unpredictable mild stress was used to establish the rat model of stressed depression. The expression of Brd U,Neu N,brain-derived neurotrophic factor( BDNF) and N-methyl-D-aspartate receptor1( NR1) in hippocampal dental gyrus were detected by fluorescently labeled immunohistochemical method.In addition,BDNFmRNA was detected by in situ hybridization. Results Chronic stress could inhibit the proliferation of neural precursors in hippocampal DG( P < 0. 01); the expression of BDNF decreased significantly in DG in model rats( P < 0. 01),while the expression of NR1 increased significantly( P < 0. 01).JWSNS and Fluoxetine hydrochloride significantly enhanced the amount of new proliferating cells and the number of neurons in unit area of DG( P < 0. 01),increased the expression of BDNF( P < 0. 01) and decreased the expression of NR1 in DG( P < 0. 01). Conclusion JWSNS could promote the neuronal proliferation in hippocampal DG of rat with chronic stressed-depression,and may exert an effect of promoting the proliferation of neurons in hippocampal DG by enhancing the expression of BDNF and decreasing the expression of NR1.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2016年第4期569-574,共6页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No30500660)
