摘要
目的研究血清溶血卵磷脂(LPC)水平在非酒精性脂肪性肝病(NAFLD)进展中的变化及机制。方法雄性C57/BL6小鼠60只,分为高脂模型组(HFD)和正常对照组,分别给予高脂纯化饲料和低脂纯化饲料。高效液相色谱-蒸发光散射检测法(HPLC-ELSD)于饲养第4、8、12、16、20周分批测定两组小鼠血清中几种LPC的含量变化。Milliplex多因子检测法测定血清白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)水平。重组白细胞介素-6(IL-6)体外干预小鼠肝原代细胞,实时荧光定量PCR测溶血卵磷脂酰基转移酶1-4(LPCAT1-4)的基因表达。结果高脂模型组血清LPC16∶0、LPC18∶0和LPC18∶1水平从16周开始出现明显降低,第20周LPC16∶0和LPC18∶1的水平均显著低于正常对照组。高脂模型组血清IL-6水平在第16周和20周显著高于正常对照组;TNF-α水平在第16周显著高于正常对照组。此外,IL-6可诱导LPCAT1-4基因表达升高。结论在NAFLD进展中血清溶血卵磷脂(LPC)水平下降,可能与肝脏内激活的炎症信号通路有关。
Objective To investigate the serum variation of lysophosphatidylcholine（LPC）concentration and relevant mechanism in the progression of non-alcoholic fatty liver disease（NAFLD）. Methods Sixty male C57 / BL6 mice were divided into high-fat group and control group. The mice in high-fat group and control group were fed with high fat diet（HFD）and low fat diet（LFD）, respectively. Serum LPC levels were measured by HPLC-ELSD at 4, 8, 12, 16 and 20 weeks. Serum interleukin-6（IL-6）and tumor necrosis factor-α（TNF-α）were determined by Milliplex kit. Primary hepatocytes were treated with recombinant IL-6. The expression of lysophosphatidylcholine acyltransferase 1-4（LPCAT1-4）were detected by real-time quantitative PCR. Results Serum LPC16 ：0,LPC18 ：0 and LPC18 ：1 levels in HFD group at20 W were significantly decreased compared with those at 16 W. Serum levels of LPC16 ：0 and LPC18 ：1 in HFD group were significantly lower than those in control group at 20 W.Serum IL-6 levels in HFD group were significantly higher than those in control group at 16 W and 20 W. Serum TNF- α level in HFD group was significantly higher than that in control group at 16 W. Furthermore, IL-6 induced the expression of Lpcat1-4 m RNA in primary hepatocytes. Conclusions Serum LPC levels decrease in the progression of NAFLD, likely due to enhanced hepatic inflammatory signaling.
出处
《热带医学杂志》
CAS
2016年第2期136-139,144,F0002,共6页
Journal of Tropical Medicine
基金
国家重点基础研究发展计划(2012CB517506)
