摘要
目的通过检测冠状动脉粥样硬化患者细胞因子信号转导抑制剂1（SOCS1）基因多态性的表达及外周血中相关细胞因子的含量，来探讨SOCS1与细胞因子及左心室射血分数的关系及其在冠状动脉粥样硬化性心脏病发生、发展中的作用及临床意义。方法选择2012年1月～2014年12月新疆医科大学第一附属医院收治的冠心病患者196例为研究组，选择同期196名健康志愿者作为对照组。利用聚合酶链反应及单核苷酸多态性分析两组SOCS1基因的多态性，同时利用酶联免疫吸附测定（ELISA）法检测血浆中自介素-1β（IL-1β）、肿瘤坏死因子仪（TNF-α）、胱抑素C（Cys-C）、同型半胱氨酸（Hcy）的水平。结果研究组和对照组rs15677380、rs173516427等位基因频率比较差异有统计学意义（P〈0．05），rs15677380C等位基因和rs173516427G等位基因均为冠状动脉粥样硬化性心脏病的高危因素（OR=1．91、1．74），rs173516427和rs15677380不同基因型左心室射血分数和IL-1β、Cys-C差异有统计学意义（P〈0．05），其中rs173516427G／G基因型和rs15677380C／C基因型的左心室射血分数最低，IL-1β、Cys-C水平最高。其余各组间比较差异无统计学意义（P〉0．05）。结论SOCS1与冠状动脉粥样硬化的发病过程有一定的相关性，可能参与其发病过程。
Objective To explore the relationship of suppressor of cytokine signaling 1 （SOCS1） with cytokines and the left ventrieular ejection fraction, and the role they play in the occurrence and development of coronary heart disease by detecting the expression of SOCS1 gene polymorphism and the contents of related cytokines in peripheral blood of pa- tients with atherosclerosis of coronary artery. Methods 196 patients with coronary heart disease admitted to the First Affiliated Hospital of Xinjiang Medical University from January 2012 to December 2014 were chosen as the study group, 196 cases of healthy volunteers at the same time were chosen as control group. Polymerase chain reaction and single nueleotide polymorphism were used to analyze SOCSI gene polymorphisms of two groups, ELISA method was used to test intedeukin-1β （IL-1β）, tumor necrosis factor-α （TNF-α）, cystatin c （Cys-C） and homocysteine （Hcy） lev- els in plasma. Results There were significant differences in allele frequencies of rs15677380 and rs173516427 between study group and control group （P 〈 0.05）, rs15677380 C allele and rs173516427G alleles were the risk factors of coro- nary atherosclerotic heart disease （OR = 1.91, 1.74）. There were statistically significant differences in left ventricular e- jection fraction, IL-1β and Cys-C between rs173516427 and rs15677380 （P 〈 0.05）, among which, the left ventricular ejection fraction of rs173516427 G/G genotype and rs15677380 C/C genotype were the lowest, whereas the IL-1β and Cys-C levels of these genotype were the highest. There were no significant differences between the other groups （P 〉 0.05）. Conclusion There is a certain relationship between the SOCS1 and pathogenesis of atherosclerosis of coronaryartery, SOCS1 may take part in the pathogenesas ol disease.
出处
《中国医药导报》
CAS
2016年第8期112-115,131,共5页
China Medical Herald
基金
新疆维吾尔自治区自然科学基金资助项目(2014211C076)
