摘要
目的:研究内皮祖细胞(EPCs)对骨髓间充质干细胞(BMSCs)膜片-种植体复合体成骨能力的影响。方法:分离培养大鼠BMSCs并采用细胞膜片技术培养细胞膜片;分离培养大鼠骨髓来源EPCs;将EPCs加载于BMSCs细胞膜片上并检测相关基因;制作细胞膜片-种植体复合体并植入裸鼠皮下,8周取材,行Micro-CT检查及组织学检测。结果:体外实验显示EPCs加载于BMSCs细胞膜片后成骨相关Runx-2、ALP、BMP2和成血管相关VEGF的基因表达提高;裸鼠体内实验取材Micro-CT检查显示加载EPCs组成骨量较高,HE切片显示加载EPCs组成骨面积较多,免疫组化检测显示加载EPCs组Runx2、BMP2表达较高。结论:加载EPCs能提高BMSCs-种植体复合物的成骨能力。
Objective： To study the effects of endothelial progenitor cells（ EPCs） on the osteogenesis of bone marrow mesenchymal stem cell（ BMSCs） sheet-implant complex. Methods： EPCs were added to the BMSC sheets,and the expression of osteogenesis-related genes was examined by real time PCR. Cell sheets were wrapped around implants to construct cell sheet-implant complexes and the complexes were subcutaneously transplanted into SCID mice. The complexes were harvested 8 weeks after operation and observed by micro-CT and histological examination. Results： The BMSC sheet with EPCs showed higher expression of Runx2,ALP,BMP2 and VEGF in the in vitro test; higher bone volume ratio,greater amount of new bone tissue and higher expression of Runx2 and BMP2 in the in vivo test. Conclusion： EPCs can improve the osteogenesis of BMSC sheet-implant complex.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2016年第2期155-160,共6页
Journal of Practical Stomatology
基金
国家自然科学基金(编号:81271104)
全军医学科技青年培育项目(编号:14QNP098)
