摘要
目的:比较大鼠的骨髓间充质干细胞(BMSCs)与脂肪干细胞(ADSCs)成骨分化能力。方法:取同一大鼠髂骨及腹股沟脂肪组织,分别提取BMSCs和ADSCs,贴壁筛选培养并传代。显微镜下观察细胞形态;用P3代细胞计数法检测细胞的增殖能力;诱导成骨后进行碱性磷酸酶染色、茜素红染色以及q PCR检测成骨相关基因。结果:2种细胞均可传代至P10代,细胞形态均一,稳定;细胞增殖实验显示P3代ADSCs的增殖能力强于BMSCs;碱性磷酸酶染色及茜素红染色表明BMSCs成骨能力强于ADSCs; q PCR显示BMSCs成骨相关基因升高更为显著。结论:BMSCs和ADSCs生物学性状稳定,具有成骨分化潜能,BMSCs成骨能力强于ADSCs。
Objective:To compare the osteogenic differentiation ability of rat bone marrow mesenchymal cells(BMSCs)and adipose stem cells(ADSCs).Methods:BMSCs and ADSCs were respectively extracted from the same rat tibia and inguinal adipose tissue.The adherent cells were cultured and passaged.The morphology of the cells was observed under a microscope,the proliferative capacity of the cells was measured by cell counts,alkaline phosphatase staining and alizarin red staining were performed after osteogenesis induction;the osteogenic differentiation ability was compared between the 2 groups of cells by qPCR of osteogenic related genes.Results:Both cells could be passaged to passage 10 with uniform and stable cell morphology.Cell proliferation experiments showed that ADSCs of P3 were more proliferative than BMSCs;alkaline phosphatase staining and alizarin red staining all showed that the osteogenic capacity of BMSCs was stronger than that of ADSCs;qPCR results showed that the expression of osteogenesis related genes in BMSCs was higher than that in ADSCs.Conclusion:BMSCs and ADSCs have stable biological characteristics,the osteogenic differentiation potential of BMSCs is stronger than that of ADSCs.
作者
卓丽丹
冯顶丽
芦笛
郭红延
李红
ZHUO Lidan;FENG Dingli;LU Di;GUO Hongyan;LI Hong(121000,Graduate School of Jinzhou Medical University,China;Shanxi Medical University School and Hospital of Stomatology,Taiyuan;General Hospital of People’s Liberation Army First Medical Center,Beijing;General Hospital of People’s Liberation Army Third Medical Center,Beijing;Beijing Military Medical Research Institute)
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2018年第6期730-735,共6页
Journal of Practical Stomatology
基金
国家自然科学基金(编号:81571619)
国家自然科学基金国际(地区)合作与交流项目(编号:313101087)
国家重点研发计划(编号:2016YFE0204400)
