摘要
目的:在基因水平上观察BMP2在肝组织的表达和肝再生过程中的动态变化特征,并结合NF-κB在肝再生过程中的表达变化特征,探讨BMP2对肝再生的影响.方法:健康成年♂Wistar大鼠54只,随机分为3组:正常对照(NC)组,即0h组(n=6),假手术(SO)组(n=24),肝部分切除术(PH)组(n=24).采用原位杂交和免疫组化的方法分别测定大鼠再生肝脏组织中BMP2 mRNA和NF-κB的表达.结果:BMP2 mRNA表达在SO组与PH组6、12、24h时间点组间存在统计学差异(灰度值:99.74±6.85vs114.41±5.12,130.59±6.74,113.74±7.32,均P<0.05).SO组与NC组无统计学意义.NC组肝组织内NF-κB阴性,SO组术后各时相点未见特异性染色.PH组NF-κB最初少量弱阳性表达,术后6、12、24h,NF-κB表达逐步增强,灰度值降低(96.22±3.12,89.59±3.24,83.72±4.32),有统计学差异(P<0.05).结论:BMP2在正常大鼠肝组织中有表达且在肝再生过程中BMP2表达呈现先减弱后增强的趋势.NF-κB在正常大鼠肝脏组织中无表达,在肝再生过程中NF-κB表达逐渐增强.BMP2可能抑制肝再生.
AIM： To analyze the BMP2 mRNA in liver dynamic changes of regeneration process, and to investigate the effect of BMP2 mRNA on liver regeneration in combination with the characteristics of nuclear factor kappa B （NF-kB） expression. METHODS： Fifty-four healthy adult male Wister rats were randomly divided into 3 groups： normal control group （NC, n = 6）, sham operation group （SO, n = 24）, partial hepatectomy group （PH, n = 24）. Using in situ hybridization and immunohistochemistry method, we measured the expression of BMP2 mRNA and NF-kB in regenerated liver tissue, separately. RESULTS： BMP2 mRNA was significantly different between the SO and PH group at 6, 12 and 24 （gray values： 99.74 ± 6.85 vs 114.41 ± 5.12, 130.59 ± 6.74, 113.74 ± 7.32; all P 〈 0.05）. There was no significance between the SO group and NC group. NF-kB was negative in the NC group, and there was no specific staining at various postoperative time points in the SO group. NF-kB was weakly positive at the initial stage in the PH group, and it was increased at 6, 12 and 24 h （gray values： 96.22 ± 3.12, 89.59 ± 3.24, 83.72 ± 4.32） （P 〈 0.05）. CONCLUSION： BMP2 is expressed in normal liver tissue of rats, and it displays a trend of increase after an initial decrease in hepatic regeneration. NF-kB is not expressed in normal liver tissue, but its expression gradually increases in the process of liver regeneration. Therefore, BMP2 may inhibit liver regeneration.
出处
《世界华人消化杂志》
CAS
北大核心
2008年第22期2453-2457,共5页
World Chinese Journal of Digestology
基金
山西省归国留学人员基金资助项目
No.200568~~
关键词
肝再生
骨形成蛋白
核因子-ΚB
原位杂交
Liver regeneration
Bone morphogenetic protein
Nuclear factor-kappa B
In situ hybridization
