摘要
采用匀浆法浸提莲藕中的多酚,以多酚提取得率和提取液DPPH自由基清除率为指标分别优化浸提工艺参数,并比较分析提取液的酚类物质组成及抗氧化活性。结果表明:以多酚提取得率为指标的优化工艺A参数为酸醇比2278(VV)、均质时间6.8 min和均质转速10 500r/min;而以提取液DPPH自由基清除率为指标优化所得工艺B参数为酸醇比1981(VV)、均质时间8.6 min和均质转速9 100r/min;各因素对多酚提取得率和DPPH自由基清除率的影响均为酸醇比>均质时间>均质转速;工艺A的多酚提取得率(165.85 mg GAE/100g·FW)显著高于工艺B(P<0.05),其DPPH自由基清除IC50值分别为4.75,6.83μg GAE/mL。工艺A较适用于莲藕抗氧化活性多酚的提取制备。
To establish the extraction technology of polyphenols from lotus root,the polyphenol yield and DPPH free radical scavenging rate were investigated to optimize the technological parameters.Then,the polyphenol composition and antioxidant activity of extracts were comparatively analyzed.The parameters of technology A optimized by polyphenol extraction yield were as follows：volume ratio of hydrochloric acid to methanol was 2278（VV）,homogenization time was 6.8 min and homogenization speed was 10 500r/min.Those of technology B optimized by DPPH free radical scavenging rate were as follows：volume ratio of hydrochloric acid to methanol was 1981（VV）,homogenization time was 8.6min and homogenization speed was 9 100r/min.The effects of experimental factors on polyphenol extraction yield and DPPH free radical scavenging rate could be both ordered as：hydrochloric acid to methanol homogenization time homogenization speed.Technology A showed a higher extraction yield（165.85 mg GAE/100g·FW）than technology B（P0.05）,and their IC50 values of DPPH free radical scavenging were 4.75 and 6.83μg GAE/mL,respectively.The results indicate that technology A better fits the extraction of antioxidant polyphenols from lotus root.
出处
《食品与机械》
CSCD
北大核心
2016年第2期128-132,共5页
Food and Machinery
基金
湖北省科技支撑计划项目(编号:2015BBA203)
关键词
莲藕
多酚
匀浆浸提
组成
抗氧化
lotus root
polyphenols
homogenization extraction
composition
antioxidant activity
