摘要
对海洋内生真菌进行化学筛选和生物活性筛选,并对由不同碳源、氮源、生长因子培养基发酵得到的样品进行评估。采用高效液相色谱法对18株海洋内生真菌发酵产物的乙酸乙酯提取物进行分析,并进行体外抗氧化和抗菌活性筛选。结果表明,样品150103(培养基1)乙酸乙酯提取液在浓度10mg/mL时对枯草芽孢杆菌和金黄色葡萄球菌有强烈的抑制效果,抑菌圈直径分别为12,11mm;对大肠杆菌无明显抑菌效果,且对DPPH自由基的清除率大于90%。高效液相色谱分析结果显示,样品150103(培养基1)提取物所含的次生代谢产物种类较多,含量丰富。样品150103(培养基1)提取物所含次生代谢产物较多、含量较大,且在体外具有较强的抗菌、抗氧化活性,值得深入研究。
A total of 18 strains of marine endophytic fungi were fermented by three fermentation media with significantly different carbon sources,nitrogen sources and growth factors,which was gotten from 54 fermentation products.These 54 fermentation products were evaluated through chemical screening and bioactivity screening.The ethyl acetate extract of 18 strains of marine endophytic fungus was screened by HPLC,antioxidant activity in vitro and antibacterial activits.Ethyl acetate extract（10mg/mL）of the strain 150103（medium 1）could inhibit bacillussubtilisand Staphylococcus aureus effectively.The diameter of inhibition zone is 12 mm and 11 mm respectively.It couldn＇t inhibit Escherichia coli evidently.It could remove more than 90% of DPPH free radicals.According to the HPLC results,extract of the Strain 150103（medium 1）is rich of secondary metabolites.The Strain 150103（medium 1）is rich of secondary metabolites,has strong antibacterial and antioxidant activity in vitro,and deserves deep research.
出处
《食品与机械》
CSCD
北大核心
2016年第2期41-43,74,共4页
Food and Machinery
关键词
内生真菌
次生代谢产物
抗氧化
抗菌
endophytic fungus
secondary metabolites
antioxidant
antibacterial
