摘要
为明确烟曲霉菌引起烟草霉烂病的限制因子,采用菌株Aspergillus fumigatus Q-1为研究对象,于PCR仪上测试了其菌丝体、分生孢子在不同温度下的存活能力。结果表明,该菌株的菌丝体在PDA培养基及水中于95℃处理10 min仍能存活;分生孢子在无水时于90℃处理10 min能存活,但其在水相中处理5 min时,在68℃处理温度中,孢子存活率仅为6.25×10^(-6)%;在65℃处理温度中,孢子存活率为8.75×10^(-4)%;在60℃处理温度中,仍有2.69%孢子存活。在水相中处理10 min时,在65℃处理温度中,孢子存活率为1.25×10^(-4)%;在60℃处理温度中,有0.29%孢子存活;在50℃处理温度中,有63.67%孢子存活。在50~70℃温度处理时接种孢子液的烟草叶柄10 min后,移至37℃下培养的烟草叶柄仍能出现典型的霉烂病。
In order to determine the limiting factor of Aspergillus fumigatus causing tobacco mildew rot,the heat tolerance of the fungus was studied.The survivability of mycelia and conidia of A.fumigatus Q-1 at different temperatures was tested by PCR machine.The results showed that Q-1 mycelia could survive in PDA medium and water treated at 95℃for 10 min.Conidia could survive at 90℃without water for 10 min,but at water phase for 5 min,the conidia survival rates were 6.25×10^(-6)%,8.75×10^(-4)%and 2.69%at 68℃,65℃and 60℃,respectively.After 10 min of treatment,the spore survival rates were 1.25×10^(-4)%,0.29%and 63.67%at 68℃,65℃and 50℃,respectively.After the tobacco petioles inoculated with spore suspension were treated at 50~70℃for 10 min and cultured at 37℃,the spores could still cause typical mildew on petioles.
作者
何鹏搏
李咏梅
朱自萍
何鹏飞
何月秋
吴毅歆
蔡永占
符宗伟
Munir Shahzad
王军伟
田阳阳
王云月
HE Peng-bo;LI Yong-mei;ZHU Zi-ping;HE Peng-fei;HE Yue-qiu;WU Yi-xin;CAI Yong-zhan;FU Zong-wei;MUNIR Shahzad;WANG Jun-wei;TIAN Yang-yang;WANG Yun-yue(Yunnan Agricultural University,Kunming 650201,China;Qujing Branch of Yunnan Provincial Tobacco Company,Qujing 655000,China;Hongta Tobacco(Group)Co.,Ltd.,Yuxi 653100,China)
出处
《江西农业学报》
CAS
2022年第8期93-96,共4页
Acta Agriculturae Jiangxi
基金
云南省烟草公司项目“烟草烘烤过程霉烂病发生原因及防治技术研究与应用”(2018530000242010)。
关键词
烟曲霉菌
烟草霉烂病
耐热性
烟草叶柄
Aspergillus fumigatus
Tobacco mildew rot
Heat tolerance
Tobacco petiole
