摘要
目的:通过戊四氮建立大鼠致癫模型,研究奥卡西平(oxcarbazepine,OXC)对大鼠脑组织内MMP-2与MMP-9表达的干预情况。方法:采用SD雄性大鼠(SPF级),随机分为正常组、戊四氮致癫组以及戊四氮致癫加高中低浓度奥卡西平组。正常组给予生理盐水、戊四氮组给予35mg/kg剂量,戊四氮加奥卡西平组高剂量200mg/kg、中剂量100mg/kg、低剂量组50mg/kg。记录大鼠行为学情况及大鼠疲劳仪上停留时间,应用Real-time PCR方法分析MMP-2、MMP-9 mRNA和蛋白表达量在各组间含量的差异。结果:用戊四氮制备的大鼠癫痫模型,MMP-2与MMP-9表达明显上调,在奥卡西平灌药3周起就能够显著降低MMP-2与MMP-9表达,并且呈现剂量相关性。RT-PCR结果表明正常组的MMP-9与MMP-2的mRNA与蛋白表达水平都处于较低的水平,当注射戊四氮后,MMP-2与MMP-9明显上调(P<0.01,P<0.01),当灌注不同剂量的奥卡西平之后,MMP-9与MMP-2的表达明显下调,200mg/kg奥卡西平组已接近正常组水平。结论:奥卡西平通过对大鼠脑组织内MMP-2、MMP-9表达的干预,对戊四氮致癫大鼠脑组织结构和功能具有保护作用,此保护作用具有剂量相关性。
Objective： To Study MMP- 2 and MMP- 9 expression in the brain tissue by establishing epileptic rat model using oxcarbazepine（ oxcarbazepine,OXC）. Methods： The subjects with SD male rats（ SPF grade）were randomly divided into normal group,pentylenetetrazol pentylenetetrazol epileptic group and low concentration oxcarbazepine group. Normal saline group were given pentylenetetrazole 35 mg / kg dose level. Pentylenetetrazole group were given pentylenetetrazole 35 mg / kg dose level,and concentration oxcarbazepine group were given oxcarbazepine the dose of 200 mg / kg,the dose of 100 mg / kg,the low- dose group 50 mg / kg. The residence time on the case and the fatigue behavior of rats instrument rats were recorded. MMP- 2,MMP- 9 mRNA and protein expression differences in content between the groups were analyzed by real- time PCR. Results： Epileptic rat model was established by using oxcarbazepine（ oxcarbazepine,OXC）. MMP- 2 and MMP- 9 expression were significantly upregulated in three weeks. Body weight and food intake of rats in each group did not change significantly.MMP- 2,MMP- 9 express after the application of oxcarbazepine treatment were reduced to near normal levels in the control group. The brain structure has been basically removed. RT- PCR results showed that in normal group,MMP- 9 mRNA and protein expression levels of MMP- 2 were at a low level. MMP- 2 and MMP- 9 were significantly increased（ P〈0. 01,P〈0. 01）. With the injection pentylenetetrazol,MMP- 9 and MMP- 2 expression were significantly reduced. 200 mg / kg oxcarbazepine group was close to normal levels with using different doses of oxcarbazepine. Conclusions： MMP- 2,MMP- 9 expression in the rat brain have a protective effect to brain structure and function by oxcarbazepine intervention with injecting pentylenetetrazol. This protective effect relate to dose.
出处
《黑龙江医药科学》
2016年第1期64-66,共3页
Heilongjiang Medicine and Pharmacy
