摘要
目的探讨脑出血(intracerebral hemorrhage,ICH)实验大鼠水肿周围脑组织中蛋白酶连接素-1(protease nexin-1,PN-1)、凝血酶(thrombin)、蛋白酶激活受体-1(protease activated receptor-1,PAR-1)的表达变化及其作用分析。方法选取成年健康雄性SD大鼠80只,编号后随机分为假手术组、ICH组,各40只。ICH组于右侧尾状核部注射自体动脉血方法制作ICH实验大鼠模型,分别于术后第12、24、120 h对2组大鼠的神经功能障碍程度进行评价;HE染色观察脑组织细胞中形态;Western blot分别于造模后第3、6、10、12、24、48及120 h检测2组大鼠脑组织中PN-1、thrombin、PAR-1实验指标的变化情况。结果 ICH组大鼠的神经功能障碍评分在造模后第12、48、120 h均显著的低于假手术组(P<0.05);ICH组大鼠在造模后第3、6、10、12、24、48及120 h的脑组织中PN-1、thrombin、PAR-1较假手术组均显著的增高(P<0.05);ICH组大鼠的脑组织中PN-1、thrombin、PAR-1在造模后12 h或24 h出现逐渐下降的趋势较造模后10 h或12 h(P<0.05)。结论 ICH实验大鼠血肿周围脑组织中PN-1有抑制thrombin及PAR-1过表达,引起神经损伤的作用。
Objective To investigate expression and role of protease catenin-1（ PN-1）,thrombin（ thrombin）,protease activated receptor-1（ PAR-1） in rats brain edema tissue after intracerebral hemorrhage（ ICH）. Methods Adult male SD rats 80 were randomly divided into sham group,ICH group after number,40 in each group,ICH group autologous arterial method of making a rat model of experimental ICH in the right caudate nucleus unit injection. The degree of neurological dysfunction between 2 groups was evaluated at 12 h,24 h and 120 h after post-operation. Observed the morphology of brain cells by HE staining. Changes of PN-1,thrombin,PAR-1 index in rat brain tissue at 3,6,10,12,24,48 and 120 h were detected by Western blot. Results Neurological dysfunction score ICH rats after modeling 12,48,120 h were significantly lower than the sham group（ P 0. 05）; ICH in rats after modeling 3,6,10,12,24,48 and 120 h of brain tissue PN-1,thrombin,PAR-1 compared with sham-operated group were significantly increased（ P 0. 05）; ICH rat brain tissue PN-1,thrombin,PAR-1 appeared in 12 h after modeling shwed a gradual downward trend（ P 0. 05）.Conclusion ICH hematoma surrounding brain tissue in rats PN-1 has the effect of inhibiting thrombin and PAR-1 overexpression,cause nerve damage.
出处
《中国生化药物杂志》
CAS
2015年第11期22-25,共4页
Chinese Journal of Biochemical Pharmaceutics
基金
广东省中医药管理局中医药强省课题(20121088)
关键词
脑出血
大鼠
脑组织
凝血酶抑制剂
intracerebral hemorrhage
rat
brain
thrombin inhibitors
