摘要
目的探讨免疫性血小板减少症(immune thrombocytopenia,ITP)患者外周血B细胞、成熟B细胞、滤泡辅助性T细胞及树突状细胞亚群的变化,及其相互关系及Tfh细胞在ITP发病中的作用。方法采用流式细胞术检测35例初治ITP患者及30例正常人外周血B细胞及成熟B细胞比例、Tfh细胞数量及树突细胞亚群。结果 ITP患者外周血CD19+B细胞及成熟B细胞比例分别为(13.49±9.05)%和(81.96±11.99)%,均高于对照组[(9.12±4.60)%和(75.42±12.86)%,P<0.05]。ITP患者Tfh细胞(CD4+CXCR5+ICOS+PD-1+)比例为(4.27±3.88)%,明显高于对照组(1.02±0.54)%,差异有统计学意义(P<0.01)。ITP患者浆细胞树突细胞比例为(2.06±1.65)%,明显低于对照组(5.07±3.48)%,差异有统计学意义(P<0.01)。结论 ITP患者存在Tfh细胞数量异常,可能导致B细胞比例升高,在ITP的发病机制中起重要作用。
Objective To study peripheral blood B cells, ma~＇e B cells, follicular helper T cells and dendritic cells of the patients with immune thrombocytopenia and explore the relationship between them and the role of Tfh cells on the pathogenesis of ITP. Methods The ratio of peripheral blood B cells and mature B cells, the number of Tfh cells and den- dritic cells were measured in 35 ITP patients and 30 healthy controls （HC）. Results The ratio of CD 19＋B cells was（13.49±9.05）% and that of mature B cells was （81.96±11.99）% in the ITP patients, which were higher than those in HC [（9.12± 4.60）%] and [（75.42±12.86）%, P 〈 0.05]. The ratio of Tfh cells in the ITP patients was （4.27±3.88）%, which was signifi- cantly higher than in the HC [（1.02±0.54）%, P 〈 0.01]. The ratio of plasmacytoid dendritic cells in the ITP patients[（2.06±1.65）%] was significantly less than the HC （5.07±3.48）%, P 〈 0.01. Conclusion The number of Tfh cells in the ITP pa- tients is abnormal, which may lead to proliferation of B cells and play an important role in the pathogenesis of ITP.
出处
《北京医学》
CAS
2016年第3期204-206,共3页
Beijing Medical Journal
