摘要
目的探讨人胎回肠淋巴组织的发生及IgM阳性B细胞在人胎回肠的分布、定位及发育。方法收集2003年1月至2005年12月贵阳市各医院妇产科因流产等终止妊娠的9～28周人胎回肠标本30例,男12例、女18例。采用HE染色观察人胎回肠淋巴组织发生,SABC法染色显示IgM阳性B细胞,并用BioMias29图像分析软件对免疫反应阳性细胞进行计数,有关数据作统计学分析。结果胎龄9周时少量淋巴细胞分布于回肠上皮外的间充质内,其中部分细胞表达膜表面IgM。胎龄17周时固有层内淋巴组织局部聚集形成孤立淋巴小结,小结内含有较多IgM阳性B细胞。自24周起,人胎回肠可见典型的集合淋巴小结。IgM阳性B细胞主要分布于淋巴小结,少量弥散分布在固有层结缔组织或绒毛上皮内。细胞计数显示9～12周人胎回肠IgM阳性B细胞数量为(12.80±5.72)个,随胎龄增加至25～28周人胎回肠IgM阳性B细胞达(201.30±36.35)个,各胎龄组间比较差异显著(P<0.05)。结论 24周人胎回肠壁淋巴组织结构基本发育成熟;人胎回肠具有潜在的IgM合成和释放能力,对胎儿肠道免疫功能的建立和健全起着重要作用。
Objective To study genesis of lymphoid tissue and distribution,localization and development of IgM-positive B cells in human fetal ileum.Methods Human fetus specimens from terminated pregnancies,12 males and 18 females with gestational ages of 9-28 weeks,were collected from hospitals in Guiyang,China,between Jan.2003 and Dec.2005.All pregnant women were informed of the purpose of this study and the protocol was approved by the Ethics Committee of Guiyang Medical College.HE staining was adopted to observe the genesis of lymphoid tissue in human fetal ileum.IgM-positive B cells were detected by immunohistochemical SABC staining,and counted by BioMias 29 image analysis software.Relevant data were statistically analyzed.Results In the ilea of 9-week fetus specimens,a small amount of lymphocytes were observed in the mesenchyme out of epithelia,part of which expressed membrane IgM.In the ilea of 17-week fetus specimens,lamina propria lymphocytes aggregated to form solitary lymphoid nodules with an increased amount of IgM-positive B cells.In the ilea of 24-week and older fetus specimens,typical Peyer＇s patches could be observed.IgM-positive B cells existed mainly in lymphoid nodules and sporadically in lamina propria connective tissue or chorionic epithelium.Cell counting results showed that IgM-positive B cells in human fetal ilea increased from 12.80±5.72 in 9-12 week to 201.30±36.35 in 25-28 week with statistically significant difference（P0.05）.Conclusion Lymphoid tissue of human fetal ileum wall is nearly mature at 24 weeks old.Human fetal ileum can potentially synthesize and release IgM that is important for constructing and improving fetus intestinal immunity.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2011年第5期493-496,共4页
Journal of Third Military Medical University
基金
贵州省教育厅基金(2002116)~~
