摘要
目的探讨免疫性血小板减少症(ITP)患者外周血树突状细胞(DC)亚群的变化及CD80、CD86表达量的改变,进一步检测白细胞介素(IL)-2、IL-4、IL-10及干扰素-γ(IFN-γ)细胞因子含量的变化,并回顾性分析其与地塞米松治疗疗效间的关系。方法留取肝素抗凝的60例ITP患者及10例正常对照者外周血标本,采用流式细胞术检测外周血DC亚群的分布情况,采用ELISA法检测相关细胞因子的含量。结果 ITP患者治疗前较正常对照组DC亚群DC2比例升高(P<0.05),治疗后与正常对照组比较差异无统计学意义;治疗前DC1、DC2上CD80的表达水平较正常对照组升高(P<0.05),DC2细胞上CD86的表达水平较正常对照组升高(P<0.05),地塞米松治疗后表达水平均下降;ITP患者治疗前较正常对照组IL-2、IFN-γ水平升高(P<0.05),地塞米松治疗后表达水平下降;IL-4、IL-10治疗前水平下降(P<0.05),地塞米松治疗后表达水平上升。结论 DC数量与功能的紊乱及IL-2、IL-4、IL-10、IFN-γ的水平变化均参与了ITP的发病,其与地塞米松的疗效可能有重要的联系。
Objective To investigate the changes of dendritic cell subsets and CD80,CD86 expression in peripheral blood in patients with immune thrombocytopenia（ ITP）,and to evaluate the changes of serum interleukin-2（ IL-2）,serum interleukin-4（ IL-4）,serum interleukin-10（ IL-10） and interferon-γ（ IFN-γ） before and after dexamethasone treatment,overall,further analyzing the relationship between them and dexamethasone. Methods Collecting blood samples of 60 ITP patients and 10 normal controls with heparin anticoagulation,and distribution of dendritic cell subsets of ITP and normal controls was detected by flow cytometry,and the changes of serum IL-2,IL-4,IL-10 and IFN-γ were detected by enzyme linked immunosorbent assay（ ELISA）. Results The percentage of DC2 was increased in ITP patients compared with the control group（ P〈 0. 05） and there was no statistically significont difference after dexamethasone treatment; the expressions of CD80 on DC1 and DC2 were increased compared with the control group（ P 〈0. 05）,as well as the expression of CD86 on DC2 and the percentage of them decreased after treatment. The serum IL-2,IFN-γ levels before the dexamethasone treatment were higher than the normal control group（ P〈0.05）,then decreased after treatment. However,the serum IL-4,IL-10 levels were lower than the normal control group before the treatment（ P〈0.05）,increased after treatment. Conclusion The disorder of the number and function of DCs and the changes of the serum IL-2,IL-4,IL-10 and IFN-γ levels may play important roles in the pathogenesis of ITP. Moreover,there is a probable key relationship between them and the effects of dexamethasone treatment.
出处
《安徽医科大学学报》
CAS
北大核心
2016年第7期998-1001,共4页
Acta Universitatis Medicinalis Anhui
基金
安徽省自然科学基金(编号:1208085MH154)
