摘要
目的：探讨磷酸化组蛋白 H3（PHH3）和细胞周期蛋白 D1（CyclinD1）在乳腺浸润性导管癌组织中的表达及意义。方法应用免疫组化 MaxVision 法检测 PHH3和 cyclinD1在89例乳腺浸润性导管癌和30例正常乳腺组织中的表达，分析其表达与乳腺浸润性导管癌临床病理参数及预后的关系。结果PHH3在乳腺浸润性导管癌和正常乳腺组织中的阳性率分别为76．4％（68／89）和6．7％（2／30），差异有统计学意义（χ^2＝45．051，P ＜0．05），PHH3过表达与病理分级（χ^2＝10．540，P ＜0．05）、腋窝淋巴结转移（χ^2＝6．852，P ＜0．05）、临床分期（χ^2＝5．634，P ＜0．05）密切相关，与患者年龄（χ^2＝1．054，P ＞0．05）、肿瘤大小（χ^2＝0．014， P ＞0．05）无关。CyclinD1在乳腺浸润性导管癌和正常乳腺组织中的阳性率分别为69．7％（62／89）和10．0％（3／30），差异有统计学意义（χ^2＝32．223，P ＜0．05），CyclinD1过表达与病理分级（χ^2＝13．328，P ＜0．05）、腋窝淋巴结转移（χ^2＝5．041，P ＜0．05）、临床分期（χ^2＝4．419，P ＜0．05）密切相关，与患者年龄（χ^2＝0．500，P ＞0．05）、肿瘤大小（χ^2＝1．836，P ＞0．05）无关。PHH3和 CyclinD1在乳腺浸润性导管癌表达呈正相关（r ＝0．497，P ＜0．05）。Kaplan-Meier 分析结果显示，PHH3的表达与预后呈负相关，用 Log-Rank 统计方法判断其中有统计学意义（χ^2＝10．468，P ＜0．05），CyclinD1的表达与预后呈负相关，用 Log-Rank 统计方法判断其中有统计学意义（χ^2＝7．906，P ＜0．05）。结论PHH3和 CyclinD1在乳腺浸润性导管癌组织中的过度表达，可能在乳腺癌的发生、发展过程中起重要作用，两者联合检测可为乳腺癌的预后评估及治疗提供理论依据。
Objective To investigate the association between expression of PHH3 and CyclinD1 and their significance in breast invasive ductal carcinoma.Methods Immunostoemical MaxVision staining was used to detect the expression of PHH3 and CyclinD1 in 89 samples of breast invasive ductal carcinoma and 30 samples of normal breast tissues.The correlation was then analyzed between the expression of PHH3 and CyclinD1 and the clinical path-ological parameters as well as prognosis.Results The positive rate of PHH3 in breast invasive ductal carcinoma tis-sues（68 /89,76.4%）was significantly higher than that in normal breast tissues（2 /30,6.7%）（χ^2 =45.051,P 〈0.05）.The expression level of PHH3 was significantly correlated with tumor histological degree（χ^2 =10.540,P 〈0.05）,anillary lymph node metastasis（χ^2 =6.852,P 〈0.05）and TNMstage（χ^2 =5.634,P 〈0.05）,but it was not related to the age（χ^2 =1.054,P 〉0.05 ）and the tumor diameter（χ^2 =0.014,P 〉0.05 ）.The positive rate of CyclinD1 in breast invasive ductal carcinoma tissues（62 /89,69.7%）was significantly higher than that in normal breast tissues（3 /10,10.0%）（χ^2 =32.223,P 〈0.05）.The expression level of CyclinD1 was significantly correlated with tumor histological degree（χ^2 =13.328,P 〈0.05）,anillary lymph node metastasis（χ^2 =5.041,P 〈0.05）and TNMstage（χ2 =4.419,P 〈0.05）,but it was not related to the age（χ^2 =0.500,P 〉0.05）and the tumor diameter （χ^2 =1.836,P 〉0.05）.The expression of PHH3 was directly correlated to the CyclinD1 （r =0.497,P 〈0.05）. Kaplan-Meier analysis showed that the expression of PHH3 was negatively correlated with prognosis.Log-Rank test indicated that there was significant difference（χ^2 =10.468,P 〈0.05）,CyclinD1 was negatively correlated with prog-nosis.Log-Rank test indicated that there was significant difference （χ^2 =7.906,P 〈0.05 ）.Conclusion High expression of PHH3 and CyclinD1 may be correlated with evolution and metastasis of breast inva
出处
《中国基层医药》
CAS
2016年第4期492-496,I0001,共6页
Chinese Journal of Primary Medicine and Pharmacy
