摘要
背景:组织工程支架材料已被广泛用于各种组织和神经修复,但是都有其局限性,效果不理想。目的:构建一种能促进脊髓损伤修复的组织工程支架材料,以期用于脊髓损伤的再生和修复。方法:乳化-溶剂挥发法制备地塞米松微球,以包封率、载药量以及收率的综合评分为指标,通过正交试验考察投药量、聚乳酸-羟基乙酸共聚物用量和聚乙烯醇质量分数对地塞米松缓释微球处方工艺的影响,扫描电镜观察微球表征。采用静电纺丝技术,以胶原蛋白和聚己内酯为原料制备复合地塞米松微球的纤维纳米支架,小鼠骨髓间充质干细胞与支架共培养3 d,扫描电镜观察细胞形态。复合材料植入大鼠脊髓缺损。结果与结论:地塞米松缓释微球最佳制备工艺为投药量10 mg,乳酸-羟基乙酸共聚物用量80%,聚乙烯醇质量分数0.5%。微球外观圆整,表面光滑;微球的载药量、包封率和收率分别为(2.26±0.03)%,(83.62±0.21)%和(90.87±2.45)%。小鼠骨髓间充质干细胞在复合地塞米松微球的材料生长状况良好。动物实验结果显示,材料与宿主无免疫反应,且随着时间的延长材料逐步降解。说明复合地塞米松微球支架材料生物相容性良好,是一种良好的生物支架材料。
BACKGROUND： Tissue engineering scaffold materials have been widely used in all kinds of tissue and nerve repair, but there are many limitations and the effect is not good. OBJECTIVE： To construct a kind of tissue engineering scaffold material for the regeneration and repair of spinalcord injury. METHODS： The dexamethasonemicrospheres were prepared by emulsification-solvent evaporation. The comprehensive scores of encapsulation efficiency, drug-loading rate and yield were taken as the indexes. The effect of dosage of dexamethasone and polylactic acid-glycolic acid copolymer and mass fraction of polyvinyl alcohol on formulation process of dexamethasone sustained-release microsphere was inspected by orthogonal experiment. The characterization of microspheres was observed by scanning electron microscope. The nanofiber scaffold of compound dexamethasone microspheres was prepared by taking collagen protein and polycaprolactone as raw materials using electrospinning technology. The mouse bone marrow mesenchymal stem cells were co-cultured with the scaffold for 3 days. Cell morphology was observed by scanning electron microscope. Composite material was implanted into the defect of spinal cord in rats. RESULTS AND CONCLUSION： The optimal preparation process of dexamethasone sustained-release microspheres： dosage of dexamethasone was 10 mg, dosage of poly lactic acid-glycolic acid copolymer was 80%, mass fraction of polyvinyl alcohol was 0.5%. Appearance of dexamethasone microspheres was smooth, with a round surface. The encapsulation efficiency, drug-loading rate and yield of microspheres were（2.26±0.03）%,（83.62±0.21）% and（90.87±2.45）% respectively. The growth of mouse bone marrow mesenchymal stem cells was good on the surface of compound dexamethasone microspheres. There was no immunological reaction between the implant material and host, and the material was degraded gradually with time. These results demonstrate that the compound dexamethasone microsphere scaffold has good biocompatibility, whi
出处
《中国组织工程研究》
CAS
北大核心
2016年第3期402-407,共6页
Chinese Journal of Tissue Engineering Research
基金
甘肃省科技重大专项(1203FKDA036)~~
