摘要
目的探讨FOXP3 m RNA荧光定量PCR技术在继发性肺结核患者疗效监测的应用意义。方法研究组选取继发性肺结核病人47例,收集初诊痰液,染色和镜检。选取健康对照40例。分离两组淋巴细胞,利用FOXP3 m RNA定量PCR技术分析对照组和研究组0周及治疗后2周、4周和8周时的FOXP3 m RNA表达情况,应用SPSS16.0软件统计分析实验数据。结果 0周时47名研究组抗酸染色均为阳性,对照组均为阴性。以抗酸染色结果分组,抗酸(+)组、抗酸(++)组、抗酸(+++)组和抗酸(++++)组与对照组FOXP3 m RNA表达差异均有统计学意义(P<0.05,P<0.01)。治疗时间对抗酸染色各组FOXP3 m RNA表达水平有影响(P<0.01),治疗时间和抗酸各组因素存在交互作用(P<0.01)。研究组和对照组FOXP3 m RNA表达差异有统计学意义(P<0.01)。研究组治疗不同时间点方差分析显示治疗时间对研究组FOXP3 m RNA表达水平有影响(P<0.01)。结论 FOXP3 m RNA定量PCR检测敏感,特异,可以用于继发性肺结核患者疗效和其机体免疫力的监测。
Objective To evaluate FOXP3 m RNA fluorescence quantitative PCR technology in the application ofcurative effect and immune system in secondary pulmonary tuberculosis patients. Methods To select 47 patients ofsecondary pulmonary tuberculosis as research group and 40 healthy donors as control group.To collect sputum from researchgroup,and then sputum smear were observed by staining and microscopy. Fluorescence quantitative PCR technology wasestablished by quantitatively analyzing the specificity of FOXP3 m RNA expression of control group at week 0 and researchgroup after treatment at week 0,week 2,week 4 and week 8. The experimental data was analyzed by using SPSS 16.0 software.Results The results of acid fast stain in sputum of research group were positive by smear microscopy,however the results ofcontrol group were negative.The difference between the FOXP3 m RNA expression of control group and that of acid fast stain（＋）group,（＋＋）group,（＋＋＋）group and（＋＋＋＋）group by smear microscopy respectively had statistical significance（P〈0.05,P〈0.01）.The FOXP3 m RNA expression of acid fast stain（＋）group,（＋＋）group,（＋＋＋）group and（＋＋＋＋）group by smearmicroscopy were influenced by time of therapy（P〈0.01）.The treatment time and factors of acid fast stain groups were withinteracted（P〈0.01）. The FOXP3 m RNA expression between control group and research group had statistical significance（P〈0.01）.The FOXP3 m RNA expression was influenced by time of therapy,using One- Way ANOVA analysis（P〈0.01）.Conclusion FOXP3 m RNA fluorescence quantitative PCR technology has high sensitivity and specificity in monitoringsecondary pulmonary tuberculosis therapeutic efficacy and immunity of patients.
出处
《中国热带医学》
CAS
2016年第2期133-136,共4页
China Tropical Medicine
基金
2015年深圳市科技研发资金项目第二批基础研究项目(No.JCYJ20150325103202291)
关键词
继发性肺结核
FOXP3
m
RNA
表达
疗效监测
Secondary pulmonary tuberculosis
FOXP3 m RNA
Expression
Curative effect monitoring
