摘要
目的:观察通督调神、疏肝解郁两种针刺方法对抑郁模型大鼠行为学及下丘脑-垂体-肾上腺轴(HPA轴)的影响,探讨二者抗抑郁作用及其机制差异。方法:SD雄性大鼠随机分为正常组和模型组。模型组采用慢性不可预见性温和性应激(CUMS)结合孤养方法造模,2周后筛选出模型制备成功的大鼠,再随机分为模型组、西药组、通督调神组、疏肝解郁组。各治疗组接受有关治疗,6日/周,持续3周。每隔7日进行1次行为学检测,3周后用放免法检测HPA轴相关指标。结果:1造模14日及治疗3周末,与正常组比较,模型组大鼠体重、旷场实验得分、糖水消耗百分比均明显降低(P<0.05),表明模型成功而稳定。2治疗3周末,两针刺组体重、旷场实验得分、糖水消耗百分比较模型组均明显增加(P<0.05)。除通督调神组旷场实验水平得分高于疏肝解郁组(P<0.05)外,两针刺组各项行为学指标间无统计学差异。3治疗3周末,模型组大鼠下丘脑促肾上腺皮质激素释放激素(CRH)、垂体促肾上腺皮质激素(ACTH)及肾上腺皮质酮(CORT)含量较正常组均明显增加(P<0.05),两针刺组较模型组均明显降低(P<0.05),且其间无统计学差异。结论:通督调神与疏肝解郁法均能明显改善抑郁大鼠的行为学指标,其中通督调神法略优;二者均能够调节HPA轴的功能亢进且作用相当,这可能是其抗抑郁的机制之一。
Objective： To observe the influences of Tongdu Tiaoshen method and Shugan Jieyu method on the changes of behavior and hypothalamus- pituitary- adrenal axis in CUMS depressive model rats,and to investigate the differences of two acupuncture methods on the antidepressant function and therapeutic effect. Methods：SD male rats were randomly divided into a normal group and a model group. Depressive model rats were established by using chronic unpredictable mild stress stimulation combined with lonely breeding. After two weeks,the successful depressive model rats were chosen and randomly divided into a model group,a western medicine group,a Tongdu Tiaoshen group and a Shugan Jieyu group. Three treatment groups received relevant treatments for 3 weeks,6 times per week. The rat＇s behavior was observed once a week and related hormones of hypothalamus- pituitary- adrenal axis were detected by radioimmunoassay techniques after 3 weeks. Results： Compared with the normal group,the body weight,consumption of sucrose and scores of open- field test of the model group declined significantly on the 14 th day after making models and after 3 weeks of the treatment（ P 〈0. 05）. It was shown that the models were duplicated successfully and stably. Compared with the model group,the body weight,consumption of sucrose and scores of open- field test of two acupuncture groups were significantly increased after 3 weeks of the treatment（ P 〈0. 05）. The result also showed that Tongdu Tiaoshen group was significantly higher than Shugan Jieyu group on the level movement scores of the open- field test（ P 〈0. 05）,but other behavioural indicators showed no statistical differences. On the three indexes of CRH hypothalamus,pituitary ACTH and adrenal CORT contents,the model group showed no significant increase compared with the normal group after 3 weeks of the treatment（ P 〈0. 05）,but the two acupuncture groups were significantly lower than the model group and there was no statistical difference between two acupuncture
出处
《针灸临床杂志》
2016年第2期64-68,共5页
Journal of Clinical Acupuncture and Moxibustion
关键词
抑郁症
通督调神法
疏肝解郁法
行为学
HPA轴
Depression
Tongdu Tiaoshen therapy
Shugan Jieyu method
Behavioral activities
HPA axis
