摘要
目的测定复发性生殖器疱疹(RGH)患者治疗前后的外周血T淋巴细胞亚群、NK细胞表达水平及s HLAG水平,了解患者治疗前后的免疫状态改变。方法采用酶联免疫吸附法和流式细胞仪分别检测60例RGH患者和42例健康对照组血浆s HLA-G水平及外周血T淋巴细胞亚群、NK细胞表达水平;采用相同的方法检测60例RGH患者口服盐酸伐昔洛韦和匹多莫德片4个月后血浆s HLA-G水平及外周血T淋巴细胞亚群、NK细胞表达水平。结果 RGH患者CD+4细胞数量低于健康对照组;血浆s HLA-G水平高于健康对照组;RGH患者治疗后CD+3T细胞(总T淋巴细胞)、CD+4T细胞、CD+8T细胞、NK细胞的数量均高于治疗前的水平,血浆s HLA-G水平治疗后低于治疗前。结论 RGH患者存在免疫系统功能紊乱,抗病毒联合免疫调节剂治疗可以予以纠正。
Objective To detect the level of the T lymphocyte subsets and NK cells in peripheral blood and the level of sHLA-G in patients with recurrent genital herpes （RGH）before and after therapy, and to learn about the change of the immune sta- tus before and after therapy. Methods The enzyme linked immunosorbent assay （ELISA） was used on the 60 cases of patients with RGH and the flow cytometry （FCM） was used on the 42 cases of healthy control to detect the level of sHLA-G and the level of T-lymphocyte subpopulation and NK cells in peripheral blood. The same method was used to detect the level of sHLA-G in plasma and the level of T-lymphocyte subpopulation and NK cells in peripheral blood in patients with RGH taking oral valaciclovir hydro- chlordide and Pidotimod tablets. Results The CD4＋ level of RGC group was lower than that of normal control group. The plasma sHLA-G level of RGC group was higher than that of normal control group. The level of CD3 cells,CD4 cells,CD8 cells and NK cells in patients with RGC were higher after therapy, and the plasma sHLA-G level is lower. Conclusion There is the dysfunc- tion in immune system in patients with RGC and therapy with antiviral drugs and immunomodulators can improve it.
出处
《四川医学》
CAS
2016年第1期24-27,共4页
Sichuan Medical Journal
基金
四川省教育厅重点科研项目(编号:11ZA067)
