摘要
目的检测复发性生殖器疱疹(RGH)患者不同病期外周血CD8+T细胞IFNγ和IL4的水平,分析其与病程、复发次数间的关系,探讨其在RGH发病中的作用。方法应用流式细胞仪对30例发作期、20例发作间歇期患者和15名健康志愿者外周血CD8+T细胞IFNγ和IL4的水平进行检测。结果发作期患者外周血Tc1百分率显著低于正常对照组(P<0.001),其中近半年复发≥3次者呈极显著性降低(P<0.001);Tc1/Tc2比值显著低于正常对照组(P<0.05);发作期患者的复发次数与Tc1和Tc1/Tc2比值显著相关(P均<0.05);在发作间歇期近半年复发≥3次患者外周血Tc1百分率显著低于正常对照组(P<0.05),发作间歇期患者的复发次数与Tc1显著相关(P<0.05)。结论近期反复发作患者外周血Tc1细胞水平低下和Tc1/Tc2比例失衡,Tc1细胞水平低下是潜伏单纯疱疹病毒活化和RGH复发的重要原因之一。
Objective To detect cytokine levels of IFN-γ and IL-4 secreted by peripheral CD8+T lymphocytes from patients with recurrent genital herpes(RGH) at different periods,to find out the relationship between the recurrent number,course and the two cytokines, and to investigate their roles in the pathogenesis of RGH.Methods Flow cytometric analysis was employed to study intracellular cytokines(IFN-γ and IL-4) in CD8+T lymphocytes in the peripheral blood of 30 patients with RGH at acute period,20 patients with RGH at recovery period and 15 healthy volunteers.Results Compared with healthy controls,RGH patients at acute period showed a decreased percentage of Tc1 subsets (P<0.001),especially a remarkable decreased percentage of Tc1 subsets (P<0.001) among those RGH patients with recurrent number more than 3 in the recent half year; whereas a decreased ratio of Tc1/Tc2(P<0.05).The recurrent number of acute patients in the recent half year were significantly correlated with the percentage of Tc1 subsets and the ratio of Tc1/Tc2(P<0.05).A decreased percentage of Tc1 subsets was showed among the RGH patients with recurrent number more than 3 in the recent half year at recovery period in comparison with healthy volunteers (P<0.05),and it was significantly correlated with the recurrent number in the recent half year(P<0.05).Conclusion There are Tc1/Tc2 imbalance and a low level of Tc1 subsets in RGH patients who are relapsing repeatedly in the near period.The low level of Tc1 subsets may be an important cause for the recurrence of RGH and the reactivation of latent herpesvirus infection.
出处
《中国皮肤性病学杂志》
CAS
北大核心
2005年第4期206-208,共3页
The Chinese Journal of Dermatovenereology
