摘要
目的探讨生理性微电场对体外培养的人胎盘滋养细胞迁移/侵袭相关分子金属基质蛋白酶(MMPs)/组织金属蛋白酶抑制剂(TIMPs)表达的影响。方法用150mV/mm的直流微电场刺激滋养细胞,测定其迁移情况并观察形态变化。实时荧光定量PCR和Western blot检测刺激前后MMP2、MMP9和TIMP1、TIMP2基因和蛋白表达水平。结果未加电刺激的滋养细胞,其运动缓慢,迁移方向随机;在含有10%小牛血清的培养基中,150mV/mm电场刺激下滋养细胞向负极迁移,迁移速度和距离明显增加(P<0.01),胞体拉长,垂直于电场方向排列。电场刺激后,胞内MMP2 mRNA和蛋白表达水平明显升高(P<0.05),MMP9、TIMP1和TIMP2表达水平无明显变化。结论生理性直流微电场可介导滋养细胞定向迁移和排列,MMP2表达水平的上调可能与微电场促进滋养细胞迁移/侵袭功能有关。
Objective To investigate the effect of small direct-current electrical stimulation on migration and invasion related MMPs/TIMPs expression of trophoblast cells.Methods The trophoblast cells were exposed to the direct current electrical field at150mV/mm for 5and 10 hours.Cell images were recorded with continuous photographing and analyzed by image analyzer.The expression levels of MMP2,MMP9,TIMP1 and TIMP2 were measured using quantitative RT-PCR and Western blot.Results In non-electrical field culture trophoblast cells migrated slowly with random directions.Trophoblast cells cultured in media containing10% calf serum with the application of 150mV/mm direct current electrical stimulation,showed marked cathodal migration（P〈0.01）,the cell body stretched,perpendicular to the direction of the electric field.Compared with the non-electrical field stimulation controls,trophoblasts under the electrical field stimulation had the increased MMP2 mRNA and protein expression（P〈0.05）,while MMP9,TIMP1 and TIMP2had no obvious changes of mRNA or protein expressions.Conclusion Physiological direct-current electrical fields might induce directed migration and perpendicular orientation of trophoblast cells.The enhanced MMP2 expression may play an important role in the migration and invasive activity of trophoblast cells in small electrical field.
出处
《重庆医学》
CAS
北大核心
2016年第7期869-872,共4页
Chongqing medicine
基金
国家自然科学基金资助项目(30872774/H0418)
四川省卫生和计划生育委员会基金资助项目(140071)
关键词
滋养细胞
金属基质蛋白酶
组织抑制因子
迁移
侵袭
电场
trophoblasts cells
matrix metalloproteinases
tissue inhibitor of metalloproteinase
migration
invasion
electrical field
