摘要
目的研究子痫前期患者胎盘滋养细胞基质金属蛋白酶(MMP)9、2及其组织抑制物(TIMP)1、2,肿瘤转移抑制基因KiSS-1 mRNA、蛋白的表达变化及其与子痫前期发病的关系。方法采用RT-PCR、免疫印迹(western blot)法对30例正常足月妊娠妇女(正常妊娠组)和10例妊娠期高血压患者(高血压组)及27例子痫前期(子痫前期组)患者胎盘滋养细胞中MMP-9、MMP-2、KiSS-1、TIMP-1和TIMP-2基因mRNA及蛋白表达水平[均以相对吸光度(A)表示]进行检测;应用明胶酶谱分析法,检测3组妇女胎盘孵育液MMP-9、MMP-2活性。结果(1)胎盘滋养细胞浸润相关基因mRNA表达水平:子痫前期组MMP-9、MMP-2 mRNA表达水平分别为0．39±0．05和0．71±0．16,均明显低于正常妊娠组的0．78±0．11和1．63±0．31,两组分别比较,差异有统计学意义(P均<0．05)。高血压组MMP-9 mRNA的表达水平明显高于重度子痫前期患者,差异有统计学意义(P<0．05)。子痫前期组胎盘滋养细胞KiSS-1 mRNA和TIMP-1 mRNA的表达水平分别为1．97±0．21和1．11±0．18,均明显高于正常妊娠组的0．69±0．27和0．65±0．19,差异均有统计学意义(P<0．05);高血压组胎盘滋养细胞KiSS-1 mRNA表达水平低于子痫前期组,但与正常妊娠组比较,差异无统计学意义(P>0．05)。重度子痫前期患者胎盘滋养细胞TIMP-2 mRNA表达水平明显高于正常妊娠组,差异均有统计学意义(P<0．05)。(2)胎盘滋养细胞浸润相关基因的蛋白表达水平:子痫前期组MMP-9、MMP-2基因的蛋白表达水平分别为1．07±0．35和0．74±0．23,均明显低于正常妊娠组的2．43±0．92和1．48±0．78,差异均有统计学意义(P<0．05)。子痫前期组胎盘KISS-1和TIMP-1蛋白表达水平分别为2．46±0．39和1．51±0．40,均明显高于正常妊娠组的0．91±0．35和0．93±0．56,差异均有统计学意义(P<0．05)。子痫前期组胎盘滋养细胞TIMP-2蛋白表达水平与正常妊娠组及高血压组比较,
Objective To study the mRNA and protein expression of matrix metalloproteinase （MMP） 9, 2, metastasis suppressor gene KISS-1, tissue inhibitor of metalloproteinase-1 （TIMP-1） and TIMP-2 in placenta of preeclampsia patients and their relation to the pathogenesis of preeclampsia. Methods Expression of MMP-9,MMP-2, KISS-1 ,T IMP-1 ,TIMP-2 mRNAs and proteins in placenta from 27 cases of preeclampsia, 10 cases of gestational hypertension and 30 cases of normal term pregnant women was detected by semi-quantitative RT-PCR, western blotting, and enzyme activity of MMP-9 and MMP-2 was measured by gelatin zymography. Results （ 1 ） Expression of MMP-9 and MMP-2 mRNA in placenta of preeclampsia （0. 39 ±0. 05 and 0. 71 ±0. 16） was significantly lower than that in normal term pregnancy （0. 78 ±0. 11 and 1.63 ±0. 31, P 〈0. 05）. Expression of KISS-1 and TIMP-1 mRNAs in placenta of preeclampsia （ 1.97 ±0. 21 and 1.11 ±0. 18 ） was significantly higher than that in normal term pregnancy （0. 69 ±0. 27 and 0. 65± 0. 19 ） （P 〈 0.05. ）. There was no significant difference in TIMP-2 mRNA level between preeclampsia and normal pregnancy （ P 〉 0. 05 ） . （ 2 ） Expression of MMP-9 and MMP-2 proteins in preeclampsia （ 1.07 ± 0. 35 and 0.74 ± 0. 23 ） was significantly lower than that in normal term pregnancy （2. 43 ± 0. 92 and 1.48 ± 0. 78） （ P 〈 0. 05 ）. Expression of KISS-1 and TIMP-1 proteins in placenta of preeclampsia （2. 46 ±0. 39 and 1.51 ±0.40） was significantly higher than that in normal pregnancy （0. 91 ±0. 35 and 0.93 ± 0. 56）（ P 〈 0. 05 ）. There was no significant difference in TIMP-2 protein level between preeclampsia and normal pregnancy （P 〉 0. 05 ）. （3）Enzyme activity of MMP-9 and MMP-2 in placenta of preeclampsia [（2.67±0.53） gray level ·g^-1·L^-1 and （1.13 ±0.28） gray level ·g^-1·L^-1）] was significantly lower than that in placenta of normal pregnancy [ （ 8.44 ± 3.70 ） gray level·g^-1·L^-1 and （3.87 �
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2006年第8期509-513,共5页
Chinese Journal of Obstetrics and Gynecology
基金
国家自然科学基金资助项目(30271362)
