摘要
目的 评价盐酸戊乙奎醚预先给药对大鼠横纹肌溶解致急性肾损伤的影响.方法 清洁级雄性SD大鼠42只,体重200～220 g,2月龄,采用随机数字表法,将其分为3组：对照组（C组,n=6）、急性肾损伤组（AKI组,n=18）和盐酸戊乙奎醚组（PH组,n=18）.AK1组和PH组采用双后肢外侧肌肉注射50％甘油10 ml/kg的方法制备大鼠横纹肌溶解致急性肾损伤模型,C组给予等容量生理盐水.PH组于给予甘油前30 min时腹腔注射盐酸戊乙奎醚0.2 mg/kg.C组于给予生理盐水后1h时取6只大鼠,AKI组和PH组于给予甘油后1、6、24 h时分别取6只大鼠,采集下腔静脉血样,采用酶比色法检测血清BUN和Cr的浓度;取肾组织,光镜下观察病理学结果,行肾小管损伤评分,采用免疫组化法和Western blot法检测DJ-1、第10号染色体缺失性磷酸酶及张力蛋白同源的基因（PTEN）编码蛋白表达.结果 与C组比较,AKI组血清BUN、Cr浓度和肾小管损伤评分升高,肾组织DJ-1表达下调,PTEN蛋白表达上调（P＜0.05或0.01）;与AKI组比较,PH组血清BUN、Cr浓度和肾小管损伤评分降低,肾组织D J-1表达上调,PTEN蛋白表达下调（P＜0.05或0.01）.结论 盐酸戊乙奎醚预先给药可减轻大鼠横纹肌溶解致急性肾损伤,其机制与上调DJ-1表达和下调PTEN蛋白表达从而抑制细胞凋亡有关.
Objective To evaluate the effect of penehyclidine hydrochloride pretreatment on rhabdomyolysis-induced acute kidney injury （AKI） in rats.Methods Forty-two pathogen-free male SpragueDawley rats,weighing 200-220 g,aged 2 months,were randomly divided into 3 groups using a random number table：control group （group C,n =6）,AKI group （n =18）,and penehyclidine hydrochloride group （group PH,n =18）.The model of rhabdomyolysis-induced AKI was established by injecting 50% glycerol 10 ml/kg into the lateral muscle of bilateral hindlimbs in AKI and PH groups.The equal volume of normal saline was given in group C.Penehyclidine hydrochloride 0.2 mg/kg was injected intraperitoneally at 30 min before administration of glycerol in group PH.Six rats were selected at 1 h after administration of normal saline in group C,or at 1,6 and 24 h after administration of glycerol,blood samples were collected from the inferior vena cava for determination of the serum blood urea nitrogen （BUN） and creatinine （Cr） concentrations by enzymic colorimetric method.The animals were sacrificed,and kidney specimens were obtained for pathologic examination and for determination of the expression of DJ-1 and phosphatase tensin homolog deleted on chromosome 10 （PTEN） encoding protein （by immuno-histochemistry and Western blot）.The damage to the renal tubules was scored.Results Compared with group C,the serum BUN and Cr concentrations and renal tubular damage score were significantly increased,the expression of D J-1 was down-regulated,and the expression of PTEN protein was up-regulated in group AKI （P〈0.05 or 0.01）.Compared with group AKI,the serum BUN and Cr concentrations and renal tubular damage score were significantly decreased,the expression of DJ-1 was up-regulated,and the expression of PTEN protein was down-regulated in group PH （P〈0.05 or 0.01）.Conclusion Penehyclidine hydrochloride pretreatment can reduce rhabdomyolysis-induced AKI probably by up-regulating the expression of DJ-1 and down-regulating th
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2016年第1期105-108,共4页
Chinese Journal of Anesthesiology
基金
全军“十二五”医药卫生科研基金（BWS11J058）
河北省应用基础研究计划重点基础研究项目（13967702D）
全军后勤科研计划（CBJ14L013）
