摘要
目的探讨盐酸戊乙奎醚预处理对内毒素性脑损伤大鼠CD14和TLR4mRNA的影响.方法将40只Wistar大鼠随机分为生理盐水对照组(NS组),内毒素性脑损伤模型组(LPS组),低剂量戊乙奎醚干预组(PL组),高剂量戊乙奎醚干预组(PH组)4组,每组各10只,实验大鼠注射内毒素4h后处死,留取脑组织标本.光镜下观察脑组织病理变化,RT-PCR法检测CD-14和TLR4 mRNA表达,ELISA法测定TNF-a的含量.结果光镜下LPS组脑细胞损伤严重,PL组、PH组与LPS组对比脑损伤减轻.与NS组相比较,LPS组、PL组及PL组脑组织TLR4和CD14 mRNA表达水平均升高(P<0.05);与LPS组比较,PL组和PH组脑组织TLR4和CD14 mRNA表达水平均降低(P<0.05);与PL组比较,PH组TLR4和CD14 mRNA表达水平降低(P<0.05).与NS组相比较,TNF-α含量升高(P<0.05);与LPS组比较,PL组和PH组脑组织TNF-α含量降低(P<0.05);与PL组比较,PH组TNF-α降低(P<0.05).结论盐酸戊乙奎醚预处理可减轻大鼠内毒素的脑损伤,机制可能与降低CD14及TLR4mRNA的表达,阻断炎症信号传导通路,减轻炎症介质的释放有关.
Objective To investigate the protective mechanism of penehychdine hydrochloride against lipopolysaccharide induced brain injury in rats. Methods 40 Wistar rats were randomly divided into group NS,group LPS, group PL and group PH. The group NS was treated with saline. The group LPS was treated with lipopolysaccharide. The group PL was treated with intraperitoneal injection of penehychdine hydrochloride(0.1 mg/kg) while they were induced by intraperitoneal infection of LPS. The group PH, which received intraperitoneal injection of penehychdine hydrochloride(1.0 mg/kg) just after the infection of LPS. Levels of CDl4 and TLR4 mRNA in brain were determined with RT-PCR. The expression of TNF-α were detected by enzyme linked immune adsorbing analysis(ELISA). The brain pathologic structure was observed under the optical microscope.Results Group NS had normal structure. The neural cells which in group LPS were arranged disorder, not only had serious pyknosis and apoptosis in brain tissue, but also had a large number of ghost cells. Between cells, there were many loosely tissue and cell-free area. The group PL showed more apoptotic cells, the cells were arranged neat. The pyknosis and ghost cells were significantly less than group LPS. The neurons in group PH had closely-arranged, there had less apoptotic cells and pyknosis, but the extent and scope were lighter than group PL, heavier than group NS. There was significant difference on expression of CD14 and TRL4 in brain of rats among four groups. Compared with NS group, the expression of CD14 and TLR4 mRNA of brain tissues in the group LPS increased obviously(P<0.05). Compared with LPS group, the level of CD14 and TLR4 mRNA of brain tissues in the group PL and PH decreased(P<0.05). Furthermore, group PH was lower than that of group PL. Compared with NS group, TNF-α increased(P<0.05). Compared with LPS group, TNF-α decreased in brain tissue of PL group and PH group(P<0.05). Compared with PL group, TNF-α decreased in PH group(P<0.05). Conclusions Penehychdine hydrochlor
作者
马琪
张小玲
王小闯
王春亚
侯艳丽
赵玉杰
骆燕妮
郭蕾
MA Qi;ZHANG Xiaoling;WANG Xiaochuang;WANG Chunya;HOU Yanli;ZHAO Yujie;LUO Yanni;GUO Lei(Intensive Care Unit, The Second Affiliated Hospital of Xi'an Jiaotong University,Xi'an710014,China)
出处
《西部医学》
2019年第6期836-839,847,共5页
Medical Journal of West China
基金
国家自然科学基金(81670049)
