摘要
目的:研究性别对局灶性脑缺血-再灌注大鼠早期脑损伤的影响。方法:随机取雌、雄健康SD大鼠各12只,分别记为F组和M组,采用栓线法制作大鼠中动脉栓塞模型,于缺血2 h再灌注24 h后断头取脑,采用TTC染色并测量各组大鼠脑梗死体积,采用新型荧光探针H2DCF-DA监测大鼠脑内活性氧自由基(ROS)阳性细胞数目并检测平均荧光强度。结果:缺血2 h再灌注24 h内,F组和M组大鼠的死亡率分别为8.33%和16.77%,差异无统计学意义(P>0.05);TTC染色显示,F组脑梗死体积为(21.42±3.54)%,M组为(40.01±4.92)%,F组小于M组(P<0.05);H2DCF-DA染色显示,F组和M组的脑缺血半暗带区ROS阳性细胞数目分别为8.56±1.89和19.04±1.37,F组少于M组(P<0.05),平均荧光强度分别为20.58±0.89和39.25±1.41,F组少于M组(P<0.05)。结论:雌鼠缺血-再灌注损伤在短期内较轻,其机制可能与雌性大鼠脑组织抑制氧自由基能力较强有关。
Objective ： To investigate the influence of gender on early cerebral damage of rats after focal ischemia reperfusion. Methods： Twenty-four healthy SD rats were randomly divided into F and M groups, including 12 females and 12 males. The rats were subjected to MCAO for 2 h and killed after reperfusion 24 h. The cerebral infarction volume was determined by TIC staining, the intracerebral ROS positive cell number and average fluorescent intensity was monitored and detected by new fluorescent probe H2DCF-DA. Results： After 2 h ischemia and 24 h reperfusion, the rats mortality rates of F and M group were 8.33% and 16.77% respectively, the differences were not statistically significant （P 〉 0.05）. TIC staining showed that the cerebral infarction volume in F group was （21.42 ± 3.54） % while that in M group was （40.01 ±4.92） %, and the cerebral infarction volume in F group was significantly less than that in M group（P 〈0.05 ）. H2DCF-DA staining showed that cerebral ischemia half dark area of oxygen free radical positive cells number of F group 8.56 ± 1.89 was significantly less than that of M group 19.04± 1.37 （P 〈 0.05 ）. The average fluorescence intensity of ROS positive cell in F group 20.58 ±0.89 was significantly less than that of M group 39.25 ±1.41 （P 〈0.05）. Conclusion： The reperfusion injury in female rats is less serious in the short term, whose mechanism may be related to estrogen in brain of female rats inhibiting oxygen free radical.
出处
《贵阳医学院学报》
CAS
2016年第2期166-169,共4页
Journal of Guiyang Medical College
基金
国家自然科学基金(81400934)
关键词
性别因素
脑缺血
脑梗死
体积
氧自由基
gender
cerebral ischemia
cerebral infarction
volume
oxygen free radical
