摘要
以黄姜为原料,提取其中色素成分,以2,6-二叔丁基对苯酚(BHT)为对照,考察了黄姜色素成分对超氧物阴离子自由基(O_2·^-)、DPPH自由基、ABTS自由基的清除能力、抗脂质过氧化能力及还原能力。结果显示,黄姜色素对超氧物阴离子自由基(O_2·^-)的清除活性略低于BHT(IC50值为0.392mg/m L);对DPPH的清除活性(IC50值为0.334mg/m L)和对ABTS的清除活性(IC50值为0.123mg/m L)均高于BHT;在实验浓度内,黄姜色素表现出了较好的抑制脂质过氧化能力(IC50值为0.258mg/m L);此外,通过普鲁士蓝法测得色素的还原能力与BHT相当。由此可知,黄姜色素具有一定的抗氧化活性,是良好的天然抗氧化剂,可望被发展成为一种功能性食品添加剂及生物药品基料。
The extraction of pigment from Dioscorea zingiberensis. The antioxidant activity of pigment from Di- oseorea zingiberensis was investigated by measuring its scavenging ability on superoxide radicals （O2 · - ） , radicals DPPH · , radicals ABTS and its total Anti-lipid peroxidation and reducing power and reducing power with control for BHT. The results showed that the scavenging ability of pigment on superoxide radicals （O2 - ） less than BHT（ IC50 =0. 392mg/mL）, the ability to scavenge radicals DPPH and radicals ABTS better than BHT（I50 =0. 334mg/mL,IC50 =0. 123mg/mL）, in the range of test concentration, pigment presents a good anti-lipid peroxidation（ IC50 = 0. 258mg/mL）, moreover, the antioxidant capability of pigment with Prus- sian blue method is familiar with BHT. Accordingly, the pigment from Dioscorea zingiberensis has a certain antioxidant activity and is a natural antioxidants which can be exploited as a functional food additive and bio- medieinals.
出处
《西北大学学报（自然科学版）》
CAS
CSCD
北大核心
2016年第1期61-65,共5页
Journal of Northwest University（Natural Science Edition）
基金
陕西省科技厅自然科学基金资助项目(2014JQ2069)
关键词
黄姜色素
抗氧化活性
抗氧化剂
pigment extracts from yellow ginger
antioxidant activity
antioxidant activity
