摘要
目的:研究莲房原花青素(LSPC)的相对分子量分布及体外抗肿瘤作用。方法:用ESI-MS分析LSPC,MTT法检测人肺癌LETP-2、胃腺癌SGC-7901、黑色素瘤A3753种细胞增殖,采用平板集落实验测定3种癌细胞集落形成。结果:LSPC的分子量范围为290.1～1154.3,是以二聚体为主的低聚原花青素;一定浓度的LSPC对3种瘤细胞均有抑制作用,当LSPC浓度为100μg/mL作用72h时对3种瘤细胞生长抑制率分别为62.6%、49.8%、77.0%;集落形成抑制率分别为77.0%、43.2%、95.3%。结论:LSPC体外对LETP-2、SGC-7901、A3753种瘤细胞有显著的抑制作用。
Objective： To study the anti-tumor activities and the relative molecular weight distribution of proanthocyanidins from lotus seedpod （LSPC） on human lung cancer LETP-2, gastric adenocarcinoma SGC- 7901, and melanoma cells. Methods： ESf-MS was used to analyze LSPC, the proliferation and colony formation rate of three tumor cells were determined by MTT and colony formation test, Results： The contents of LSPC were mainly di-metric proanthocyaidins, and the relative molecular weight of LSPC were ranged from 290,1 to 1154.3. It was showed that the LSPC with a certainty concentration could inhibit the three tumor cells, and the inhibition rate of growth were 62.6%, 49,8% and 77.0%（P〈0.05-0,001）, respectively, and the inhibition rate of colony formation rate were 77.0%, 43,2%, 95.3%（P〈0.05-0,001）, respectively, when the concentration of LSPC was 100μg/mL and incubated for 72h, Conclusion： The results indicate that LSPC has prominent anti-tumor activities on LETP-2, SGC-7901, A375 in vitro.
出处
《食品科技》
CAS
北大核心
2007年第10期251-254,共4页
Food Science and Technology
基金
江苏省教育厅自然基金项目(05KJB550011)
江苏大学人才基金项目(05JDG013)。
关键词
莲房
原花青素
肿瘤细胞
抑制作用
Lotus seedpod
proanthocyanidins
tumor cell
inhibitory effect
