摘要
目的观察基质金属蛋白酶9(MMP-9)在肺癌中的表达情况及其与临床病理参数和预后的关系。方法利用流式细胞术(FCM)定量检测肺癌组织及正常组织和肺部良性病变中的MMP-9蛋白在细胞中的表达情况,并利用统计学方法分析其与肺癌临床病理特征和预后的关系。结果肺癌中MMP-9的表达明显高于肺正常组织和肺部良性病变组织(P<0.05)。在肺腺癌中MMP-9的表达水平高于肺鳞癌(P<0.05),且MMP-9的表达与肺癌的组织学分级及临床分期密切相关,低分化组的MMP-9表达量明显高于高分化组(P<0.05),晚期患者的MMP-9表达也明显高于早期患者(P<0.05)。另外,多因素COX回归分析表明MMP-9是影响肺癌预后的独立因素(P<0.05)。结论 MMP-9在肺癌中处于高表达状态,其表达水平与肺癌的病理类型、肿瘤分化及临床分期密切相关,且MMP-9是肺癌预后的独立影响因素。
Objective To investigate the correlation of matrix metallopeptidase 9（ MMP- 9） protein expression with clinicopathological characteristics and the prognosis in lung cancer. Methods Flow cytometry（ FCM） was utilized to examine protein expression of MMP- 9 in lung cancer tissues,normal tissues and lung benign lesion tissues. The relationship of MMP- 9 expression with clinicopathological characteristics and prognosis was statistically analyzed. Results The expression of MMP- 9 in lung cancer was significantly higher than that in normal lung tissues and lung benign lesion tissues（ P〈0. 05）. The expression level of MMP- 9 in lung adenocarcinoma was higher than that of squamous cell carcinoma of the lung（ P〈0. 05）,and the expression of MMP- 9 was closely related to the histological grade and clinical stage,MMP- 9 expression was higher in low differentiation group than that in high differentiation group（ P〈0. 05）. The expression of MMP- 9 in patients with advanced lung cancer was significantly higher than that in patients with early stage（ P〈0. 05）. In addition,Cox multivariate analyses showed that MMP-9 was an independent factor affecting the prognosis of lung cancer（ P〈0. 05）. Conclusion MMP- 9 is highly expressed in lung cancer,the expression of MMP- 9 is closely related to the histological grade,clinical stage and the pathological type of lung cancer,and MMP- 9 is an independent factor affecting the prognosis of lung cancer.
出处
《临床和实验医学杂志》
2016年第4期322-325,共4页
Journal of Clinical and Experimental Medicine
基金
2014年廊坊市科学技术研究与发展计划项目(编号2014013033)
