摘要
目的 建立高效液相色谱法同时测定赶黄草中槲皮素和山柰酚含量的方法.方法 采用高效液相色谱法,色谱柱为Kromasil C18 柱（4.0 mm×250 mm,5.0 μm）,流动相为甲醇-0.1%磷酸溶液（50∶50）,流速1.0 mL/min,检测波长360 nm,检测温度为室温.结果 槲皮素、山柰酚分别在0.105 6～2.112 0 μg 和0.023 6～0.472 μg 范围内具有良好线性关系,平均回收率分别为98.46%（RSD=2.29%）和98.17%（RSD=1.99%）.结论 该法准确、灵敏,可用于赶黄草中槲皮素和山柰酚的含量测定.
Objective To establish an HPLC method for the simultaneous content determination of quercetin and kaempferol in Penthorum Chinense Pursh. Methods The HPLC analysis was carried out on Kromasil C18 (250 mm× 4.0 mm, 5.0 μm) with a mixture of methanol-0.1% phosphate (50:50) as the mobile phase. The determination wavelength was set at 360 nm with the flow rate of 1.0 mL/min. The column temperature was set at room temperature. Results The quercetin and kaempferol showed good linearity in the range of 0.105 6–2.112 0 μg and 0.023 6–0.472 μg respectively. The average recovery rates of quercetin and kaempferol were 98.46% (RSD=2.29%) and 98.17%(RSD=1.99%) respectively. Conclusion The method is accurate and sensitive, which can be used for the content determination of quercetin and kaempferol in Penthorum Chinense Pursh.
出处
《中国中医药信息杂志》
CAS
CSCD
2016年第3期77-79,共3页
Chinese Journal of Information on Traditional Chinese Medicine
基金
中央高校基本科研业务费专项资金项目(zyz2011058)
西北民族大学科研创新团队计划(2014年)
关键词
赶黄草
槲皮素
山柰酚
高效液相色谱法
含量测定
Penthorum Chinense Pursh
quercetin
kaempferol
HPLC
content determination
