摘要
DNA的分离和分析与我们的生活息息相关.在日常生活中,医生正越来越多地用病人的基因信息来诊断疾病,法庭常常用DNA比对的方法来判定嫌疑人是否有罪,疾病控制中心的科研工作者通常会微生物的DNA图谱来判断病原体,所有这些都是基于DNA的分离和分析.目前,分离DNA最常见的方法是凝胶电泳法,该方法分离能力强分离效率高.但是,用于分离的胶的制备耗时耗力,这大大影响了DNA的分离速度.为了解决这一问题,我们首次提出了基于无涂层微纳毛细管的流体动力学色谱的方法.该方法在自由溶液中进行,无需筛分介质或者施加电场,可以实现DNA的快速高效分离.
Separation of DNA plays a critical role in our everyday life. Based on DNA analysis, a doctor increasingly uses a patient＇ s genetic information to diagnose a genetic disease, a court uses the comparison of the DNA of an accused with that found at the crime scene to decide on guilt or innocence, scientists at Centers for Diesease Control use the DNA fingerprint of a microorganism to identify the specific strain of a pathogen, and so forth. DNA molecules are usually separated using gel electrophoresis with high efficiency. However, preparation of gel is time-consuming. To address this problem, we for the first time in the world propose a novel technique, namely bare narrow capillary-hydrodynamic chromatography. This technique is performed in a free solution and no voltage is applied. It can be used for rapid DNA analysis with high efficiency.
出处
《玉林师范学院学报》
2015年第5期2-11,共10页
Journal of Yulin Normal University
关键词
无胶DNA分离
流体动力色谱
NDA筛分
高通量分析
gel-free DNA separation
hydrodynamic chromatography
DNA sizing
high-throughput analysis.
