摘要
利用芯片电泳方法考察瞬间等速电泳-筛分电泳偶联分析的结果,比较了自由溶液和筛分介质中DNA瞬间等速电泳的预浓缩效果。结果显示,相比较于筛分介质条件,自由溶液瞬间等速电泳有利于改善预浓缩和后续筛分电泳分离效果。对此结果的解释是:自由溶液条件下DNA迁移速度的提高可以延长瞬间等速电泳持续时间,有利于提高预浓缩效率。此外,样品压缩区带在自由溶液-筛分介质界面的二次富集也是预浓缩效果得到改善的原因之一。
Transient isotachophoresis （tITP） is a simplified ITP scheme for preconcentration and separation of DNA samples. The tITP preconcentration is determined by tITP time that depends on the mobility difference of the terminating ions and the sample ions as well as of the terminating ions and the leading ions. The performances of on-chip tITP DNA preconcentration in free-solution and gel buffer were studied by investigating analytical data from tITP and capillary sieving electrophoresis （CGE） coupled analysis with laser-induced fluorescence detection. The results indicated that tITP in free-solution gave higher preconcentration and separation efficiency compared to that in gel buffer. The results were explained by the theory that the higher electrophoretic mobility in free-solution resulted to increased ITP time and therefore highly efficient tITP preconcentration. In addition,a further stacking at the interface between free-solution and gel also contributed to the improvement of preconcentration effects.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2010年第8期1100-1104,共5页
Chinese Journal of Analytical Chemistry
基金
"十一五"重大专项(No.2008ZX10004-004)
国家自然科学基金(No.30870753)资助
关键词
芯片电泳
等速电泳
预浓缩
脱氧核糖核酸
Microfluidic electrophoresis
Isotachophoresis
Sample preconcentration
Deoxyribonucleic acid
