摘要
利用供试的甘蓝CMS材料对影响甘蓝未受精子房离体培养诱导的主要因素(包括外植体的基因型、外植体发育时期、外源激素类型及浓度、高温预处理温度及时间、灭菌所用次氯酸钠浓度及时间等)进行研究,旨在对各种处理条件下诱导率情况进行分析。通过单因素、多因素等试验方法筛选出能够诱导成苗的未受精子房离体培养体系,明确甘蓝未受精子房离体培养发育途径。结果表明:基因型RM的诱导效果最好;当外植体处于花初期时最易诱导成功;诱导未受精子房产生愈伤组织的适宜培养基MS+0.5 mg/L 6-BA+2.0 mg/L 2,4-D+0.1 mg/L NAA,愈伤组织及雌核发育胚总诱导率可达28.57%;诱导愈伤组织分化不定芽的适宜培养基为MS+0.1 mg/L NAA+0.2 mg/L 6-BA+0.05 mg/L TDZ,不定芽分化频率可达57.12%;将不定芽转移至1/2MS+0.1 mg/L NAA培养基中可诱导生根,发育成完整植株;33℃高温诱导1天的平均芽点数及芽点诱导率均最高,分别为2.85和100%;次氯酸钠浓度为2%、处理时间为15 min时,平均芽点数和芽点诱导率均最高,分别为2.93和97%。
In vitro culture of unfertilized ovary of cabbage（Brassica oleracea L.） is affected by many factors（genotypes, growth period, phytohormones and its concentration, temperature, Na Cl O concentration forsterilization and treated time）. This study aims to analyze the induction rate under various conditions, screenout a technique that can effectively induce unfertilized ovary to be a plant in vitro through single-factor andmultiple-factor analysis experiments, and understand the development of unfertilized cabbage ovary in vitro.The results showed that genotype RM had the best induction effect, and the explant was successfully inducedwhen it was in early flowering stage. The suitable media component for callus induction was MS＋ 0.5 mg/L6- BA ＋ 2.0 mg/L 2,4- D ＋ 0.1 mg/L NAA, 28.57% was the highest induction rate of callus and gynogenicembryos. The best and most suitable media component for adventitious bud was MS＋ 0.1 mg/L NAA＋ 0.2 mg/L6-BA＋0.05 mg/L TDZ, the regeneration frequency was up to 57.12%, adventitious buds and embryos couldroot and develop into complete plants when they were transplanted in the media（1/2MS＋ 0.1 mg/L NAA）. Theaverage bud points and bud induction rate were the highest at 33℃ for 24 h, which were 2.85 and 100%,respectively. Treated with Na Cl O of 2% concentration for 15 min, the average bud points and bud inductionrate were the highest, which were 2.93 and 97%, respectively.
出处
《中国农学通报》
2016年第1期52-58,共7页
Chinese Agricultural Science Bulletin
基金
黑龙江省教育厅科技项目"甘蓝未受精子房培养"(12541035)
