摘要
以花椰菜的花托、花序轴和未受精子房为外植体 ,诱导出不定芽 ,并进行继代增殖培养 ,在继代增殖培养基上每隔 2 5～ 30天 ,不定芽可以增殖 5～ 6倍 ,在不加激素的 1/2MS培养基上 ,试管苗生根率达到 98%以上 ,移栽成活率达到 95%以上。经观察 ,花托和未受精子房再生植株是由外植体直接分化而来 ,不经过愈伤组织阶段 ,这样可避免不良变异 。
Receptacle,rachis and unfertilized ovary of cauliflower were as explant for inducing plant regeneration and subcultured.Subcultured shoots on Ms 199 medium could be increased to 5-6 times in every 25-30 days.Rooting rate reached more than 98% on 1/2 Ms medium without any hormone.The surviving rate of the plantets transfered into soil reached more than 95%.Throngh abservation,We found that the plantlets came directly from receptacle and unfertilized ovary explants without callus stage,thus can avoid bad variation and keep genetic stability of culture materials.
出处
《长江蔬菜》
2000年第1期28-30,共3页
Journal of Changjiang Vegetables
关键词
花椰菜
未受精子房
花托
花序轴
离体培养
育种
Cauliflower
Unfertilized ovary
Receptacle
Rachis
In vitro
Regenerated plant
