摘要
以8个辣椒(Capsicum annuum L.)杂交品种的未受精子房为试材,对辣椒未受精子房在不同试验条件下的离体培养进行研究。结果表明,供试的8个品种中"辣优八号"品种愈伤组织的诱导率最高,达25%;花药发育单核靠边期的子房诱导率最高;70%酒精30 s+0.1%升汞是合适的消毒方法;适宜的愈伤组织诱导培养基为MS+0.5 mg/L 2,4-D+1.0 mg/L 6-BA,愈伤组织在MS+1 mg/L NAA+3 mg/L BA、MS+1 mg/L NAA+2 mg/L BA与MS+1 mg/L NAA+1 mg/L BA增殖培养基上均能不断增殖和膨大,3个增殖培养基间无明显差异。
The unfertilized ovaries of eight crossbreed pepper cultivars（Capsicum annuum L.） were tested to study isolated culture of the unfertilized ovaries under different experimental conditions.The results showed that the highest induction of callus from unfertilized ovaries of eight crossbreed pepper cultivars was ＂Layoubahao ＂,which was 25%,The highest induction rate of callus from ovaries with anthe occurred in late uninucleate stage,The optimum sterilization way was 70% alcohol 30s ＋ 0.1% HgCl2,The optimum culture medium of calluses inductions from unfertilized ovaries in pepper were MS ＋ 0.5 mg/L 2,4-D ＋ 1.0 mg/L 6-BA,Calluses were proliferating and enlarging in culture mediums,these culture mediums are MS ＋ 1 mg/L NAA ＋ 3 mg/L BA,MS＋1 mg/L NAA ＋ 2 mg/L BA and MS ＋ 1 mg/L NAA ＋ 1 mg/L BA,and the results from these culture mediums didn＇t differ much from each other.
出处
《西北农业学报》
CAS
CSCD
北大核心
2008年第5期310-316,共7页
Acta Agriculturae Boreali-occidentalis Sinica
基金
“十一五”国家科技支撑计划(2006BAD01A7)
西北农林科技大学植物遗传育种专项(05YZ024-1)
