摘要
研究根据草莓基因组和月季基因组的同线性关系,高通量开发月季SSR共显性标记。利用MISA软件对总长206.8Mb的草莓基因组序列进行扫描,共识别49 029个SSR位点,平均每4.2 kb包含1个SSR位点。利用primer3软件成功对21 288个SSR位点设计引物。利用中国古老月季品种‘月月粉’(Rosa chinensis‘Old blush’)和园艺品种‘无刺光叶蔷薇’(Rosa wichuriana‘Basye’s thornless’),随机选取300对SSR引物进行PCR扩增,40对引物获得清晰条带,扩增率为13.3%,发现其中8个可转移的SSR位点位于草莓基因组第六染色体63 959 bp的基因组区间。研究结果为月季和草莓间的比较基因组学研究提供了分子证据,为月季生物学研究提供宝贵的标记资源。
Focusing on high throughput SSR markers development based on the genome synteny between woodland strawberry（ Fragaria vesca） with roses,we employed Microsatellite software to scan the woodland strawberry genome sequence with 206. 8 Mb length,total 49 029 SSR loci were identified and the average distance between SSRs was 4. 2 kb. 21288 SSR primer pairs were successful designed by primer 3software. Then,we selected 300 primer pairs to validate the transferability to rose genome using Rosa chinensis‘Old blush＇and Rosa wichuriana‘Basye＇s thornless＇. As a results,forty SSR microsatellite loci successful amplified,and the rate of amplification was 13. 3 percent.In additional,we found a 63 959 bp high conservative genome region contains eight transferable SSR loci. The work present here would provide a molecular evidence for comparative genome research between Fragaria vesca with roses and a larger set of SSR markers.
出处
《西南农业学报》
CSCD
北大核心
2015年第6期2681-2685,共5页
Southwest China Journal of Agricultural Sciences
基金
国家自然科学基金项目(31160402)
云南省科技计划项目(2013FZ148)
