摘要
【目的】利用野栽杂交分离群体定位水稻结实率,为能更好地挖掘和利用野生稻中控制穗结实率基因的QTL位点提供参考。【方法】分别以广西普通野生稻资源Y03为父本和栽培稻品种日本晴为母本,经过杂交构建包含142个单株的F2定位群体,然后利用覆盖水稻基因组的184对SSR分子标记,采用复合区间作图法(CIM),以LOD=2.5为阈值检测控制结实率的QTL。【结果】共检测到3个影响结实率的QTL。其中,2个QTL位于第1染色体,1个QTL位于4号染色体上,并分别命名为q SSR1-1,q SSR1-2和q SSR4-1。q SSR1-1位于第1染色体RM486~RM5501,表型贡献率为14.49%;q SSR1-2位于第1染色体RM102~RM315,表型贡献率为8.63%;q SSR4-1位于第4染色体RM252~RM119,表型贡献率为8.27%。对结果进行分析还发现,在3个QTL位点上来源于野生稻亲本Y03的等位基因均有利于提高水稻结实率。随后,根据获得的主效QTL定位信息最终开发出与水稻结实率性状紧密连锁、可用于分子育种的分子标记RM119。【结论】发掘的新QTL和性状连锁标记可为水稻产量性状QTL的发掘和分子标记辅助选择育种提供重要的基因资源和分子选择工具。
[ Objective ] The seed setting rate of rice was determined by wild-seeded hybrid isolates, to provide a reference for using the genes/ QTLs of control seed setting rate in wild rice. [ Method]The common wild rice resources in Guangxi Y03 as the male and Nipponbare as the female parent, F2 locating population of 142 individuals were constructed by hybridization. 184 polymorphic SSR markers were used for map- ping QTLs for seed setting rate by combining MAPMAKER EXP 3.0 and composite interval mapping with LOD = 2.5. [ Result ] Three QTLs associated with seed setting rate were detected. These QTLs distributed on the chromosome 1,4. Two QTLs , which were named qSSRI-1 and qSSR1-2 respectively, were mapped on the chromosome 1. One QTL, qSSR4-1, was mapped on chromosome 4. qSSRI-1 was mapped between RM486 to RM5501 on chromosome 1, explaining 14.49 % of observed phenotypic variation, qSSR1-2 located between RM102 to RM315 on chromosome 1 and explained 8.63 % of observed phenotypic variation, qSSR4-1 was mapped between RM252 to RM119 on chromosome 4, explaining 8. 27 % of the observed phenotypic variation. The alleles for increasing seed setting rate were derived from Y03 at qSSRI-1, qSSR1-2 and qSSR4-1. Moreo- ver, SSR marker RMl19, was identified for associating with seed setting rate closely and being able to apply in rice molecu- lar breeding. [ Conclusion ] Based on the result in the study, some important genes and molecular tool would able to be pro- vided for rice molecular breeding in the future.
作者
潘英华
温国泉
徐志健
梁云涛
PAN Ying-hua WEN Guo-quan XU Zhi-jian LIANG Yun-tao(Rice Research Institute of Guangxi Academy of Agricultural Sciences, Guangxi Nanning 530007, China Guangxi Key Laboratory of Rice Genetics and Breeding, Guangxi Nanning 530007, China Agricultural Information Institute of Guangxi Academy of Agricultural Sci- ences, Guangxi Nanning 530007 ,China)
出处
《西南农业学报》
CSCD
北大核心
2017年第7期1473-1478,共6页
Southwest China Journal of Agricultural Sciences
基金
广西壮族自治区主席科技资金项目"野生稻优异基因的挖掘和利用研究"(1517-03)
广西农业科学院基本科研业务专项项目"野生稻资源保护与利用研究"(2015YT14)
国家科技基础条件平台项目"国家野生稻种质资源平台(南宁)"(NICGR2016-039)
广西农业科学院基本科研业务专项"广西药用野生稻基因MKK3的功能验证"(桂农科2017YZ08)
广西农业科学院基本科研业务专项"广西野生稻等保护点目标物种居群多样性分析"(2015JZ12403)
关键词
普通野生稻
杂交
控制穗结实率
QTL
Oryza rufipogon （
rift.
Hybridization
Control seed setting rate
QTL
