摘要
近年来,焦磷酸测序、SOLiD测序等一类新型测序技术引起了人们广泛的关注,这些技术统称为新一代测序(Next generation sequencing,NGS)技术。与第一代Sanger测序技术相比,NGS测序技术具有检测通量更高、用时更少、准确度更高以及检测费用更低等优点。序列的检测可以用于精确的鉴别微生物菌群,包括不可培养的生物体和含量极少的微生物,NGS技术的研发给微生物学研究带来了巨大的变革。通过对NGS系统和平台的介绍,综述了NGS技术在食品微生物学和食品发酵研究中的应用,并探讨了它们的局限性和发展前景,该技术的应用可以增加对微生物多样性、菌群结构和微生物数量动态变化的研究,从而提高对食品(主要是发酵食品)的监控。
In recent years, there has been an explosion of newly-developed sequencing strategies, such as pyrosequencing and SOLID sequencing, which are called Next Generation Sequencing （NGS） techniques. Compare to the first generation sequencing technology, Sanger sequencing, NGS techniques have the advantages of high-throughputs, less time, higher accuracy and lower costs. These sequences allow for the accurate identification of microbial taxa, including uncultivable organisms and those present in small numbers. NGS techniques are the revolution in the field of microbiology. This review introduces the most common NGS systems and platforms, and summarizes the application of NGS techniques in the study of food microbiology and food fermentations. Also discuss their limits and perspectives at last. The microbial diversity, population structure and population dynamics can be studied further by the application of NGS techniques. NGS techniques can also improve the monitoring and manipulation of foods （most for fermented food products）.
出处
《食品工业》
CAS
北大核心
2016年第1期278-283,共6页
The Food Industry
基金
国家自然科学基金(31271897)
黑龙江省科技计划项目(GC13B212)
关键词
新一代测序技术
食品微生物学
发酵食品
next generation sequencing
food microbiology
fermented food products
