摘要
基于Illumina MiSeq高通量测序平台,对甘南牦牛曲拉样品中细菌16S rRNA V3-V4区进行测序,通过α多样性、物种分类组成及β多样性分析对曲拉中细菌多样性及群落结构进行分析。结果表明:曲拉样品中优势门为厚壁菌门(Firmicutes)和变形菌门(Proteobacteria),优势属为乳杆菌属(Lactobacillus)、醋酸杆菌属(Acetobacter)、乳球菌属(Lactococcus),不同来源的样品中群落组成存在差异;细菌的功能基因预测表明,不同来源样品的细菌群落之间存在差异。研究结果可为曲拉的利用和食用安全性提供理论依据。
In this study,we adopted Illumina MiSeq high-throughput sequencing technology to sequence the V3-V4 region of the 16S rRNA gene of bacteria in Qula samples from the southern area of Gansu province,and analyzed the diversity and community structure of bacteria in Qula throughαdiversity,species composition andβdiversity analysis.The results showed that Qula samples from different pastoral households differed in terms of bacterial community composition.Firmicutes and Proteobacteria were the dominant phyla.Lactobacillus,Acetobacter and Lactococcus were the dominant genera.The functional genes of bacteria in the samples showed that the bacterial communities from different sources were different.The results can provide a theoretical basis for the utilization and food safety of Qula.
作者
曹磊
梁春御
曹瑛瑛
文开勇
文鹏程
杨敏
冯晓蘶
张忠明
张卫兵
CAO Lei;LIANG Chunyu;CAO Yingying;WEN Kaiyong;WEN Pengcheng;YANG Min;FENG Xiaowei;ZHANG Zhongming;ZHANG Weibing(College of Food Science and Engineering,Gansu Agricultural University,Lanzhou 730070,China;College of Meteorological,Lanzhou Resources and Environment Vocational and Technical College,Lanzhou 730021,China;Gansu Food and Drug Administration,Lanzhou 730070,China;College of Science,Gansu Agricultural University,Lanzhou 730070,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2019年第22期103-109,共7页
Food Science
基金
国家自然科学基金地区科学基金项目(31560442
31760466
31960486)
企业研究转化与产业化专项(2018-SF-C29)
甘肃农业大学学科建设专项基金项目(GAU-XKJS-2018-247)
关键词
曲拉
高通量测序
细菌多样性
Qula
high-throughput sequencing
bacterial diversity
