摘要
采用Boltzmann函数拟合结合两阶段动力学模型(成核阶段和生长阶段),研究了胶原浓度、温度、溶液p H和离子强度对罗非鱼皮Ⅰ型胶原自聚集动力学的影响。结果表明:胶原浓度、温度、溶液p H和离子强度能调控胶原自聚集动力学。当浓度达到或高于0.34 mg/m L时,胶原表现明显的自聚集,且浓度越高越有利于胶原晶核的形成,过量的胶原分子能阻碍胶原纤维的生长;聚集温度越接近胶原来源原料的生活温度或溶液p H偏碱性,胶原越易自聚集;Na Cl浓度也可影响胶原的自聚集动力学,适量浓度的Na Cl有利于胶原晶核的形成,但Na Cl浓度过高却对胶原纤维的生长有阻碍作用。
The effects of collagen concentration,temperature,p H value and ionic strength on the kinetic self-assembly of type Ⅰ collagen from tilapia（Oreochromis niloticus） skin were investigated by Boltzmann function and a two-phase kinetic self-assembly model（nucleation phase and growth phase）. It showed that the kinetic self-assembly of collagen could be modulated by collagen concentration,temperature,p H value and ionic strength. The marked self-assembly was formed when the concentration of collagen reaching and above0.34 mg/m L from the fitted kinetic self-assembly curve. More of collagen molecules were beneficial for the formation of nucleus,but excess would inhibit the growth of fibril. Collagens were more likely to self-assemble when the temperature was close to the environmental temperature for samples or in the alkaline solution.Finally,the collagen self-assembly could be modulated by Na Cl concentration. The higher Na Cl concentration was beneficial to the formation of nucleus,but the superabundant of Na Cl would inhibit the growth of fibril.
出处
《食品工业科技》
CAS
CSCD
北大核心
2016年第2期93-97,102,共6页
Science and Technology of Food Industry
基金
国家自然科学基金(31201455
41576155)
山东省海洋经济创新发展区域示范产业项目(海洋活性蛋白及其功能制品的开发与产业化)
关键词
胶原
自聚集
罗非鱼
鱼皮
动力学
collagen
self-assembly
tilapia
fish skin
kinetic
