摘要
【目的】通过扫描电镜观察壳聚糖和Ⅰ型胶原在不同温度下以不同比例制备成冻干状组织工程复合支架材料的微观结构,以获得最佳浓度、比例、温度匹配组合,制备理想的组织工程复合支架材料。【方法】在-5℃、-20℃和-80℃将不同浓度的壳聚糖、Ⅰ型胶原以及它们不同比例的混合液冷冻12h后冻干24h,扫描电镜观察不同材料表面和内部的微观结构,并计算其平均孔径和孔隙率。【结果】壳聚糖、Ⅰ型胶原以及它们的混合液冻干后都形成白色海绵状固体,冷冻温度越低冻干后材料的孔径越小,孔隙率越低;材料的浓度越高冻干后孔径越小,孔隙率越低;壳聚糖-Ⅰ型胶原混合液中壳聚糖含量越高,材料冻干后孔径越小,孔隙率越低。【结论】壳聚糖-Ⅰ型胶原复合支架的孔径和溶液浓度、二者配比以及冻干前的冷冻温度密切相关。
[Objective] To observe the various microstructures of frozen drying forms of tissue engineering scaffold, which was made of chitosan and type Ⅰ collagen with their various proportions, concentrations, and under different temperatures by scanning electron microscope （SEM）. To obtain the ideal combinant tissue engineering scaffold. [Methods] Different concentration of chitosan, type Ⅰ collagen and the mixed liquor of them in different proportions were frozen firstly for 12 h at -5, -20, and -80 ℃, respectively, then were frozen dried for 24 h, at last, the microstructure of the new materials were observed by scanning electron microscope, and the average pore diameters and interval porosities were calculated. [Results] Platinum sponge solid were formed after chitosan, type Ⅰ collagen and the mixed liquor of them were frozen drying. And the lower temperature materials were frozen before drying, the smaller pore diameter and lower interval porosity they had. The higher concentration of materials and higher proportion of chitosan in mixed liquor were, the smaller pore diameter and lower interval porosity they had. [Conclusion] The pore diameters of chitosan-type Ⅰ collagen composite scaffold are closely related with the concentration, proportion of materials and the freezing temperature before drying.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2006年第5期557-561,共5页
Journal of Sun Yat-Sen University:Medical Sciences
基金
广东省科技计划项目(2004B31201003)
关键词
壳聚糖
Ⅰ型胶原
复合支架
扫描电镜
chitosan
type Ⅰ collagen
composite scaffold
scanning electron microscope
